165,385 results

1. pMK025

   Plasmid

   #236572

   Purpose

   Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE

   Plasmid

   #21836

   Depositor

   David Ron

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   farnysylation signal

   Expression

   Mammalian

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-PE-bacteria

   Plasmid

   #172715

   Purpose

   Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   Cas9n-linker-M-MLV2
   

   Expression

   Bacterial

   Mutation

   H840A of Cas9

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLT3-ITD-MSCV-IRES-GFP

   Plasmid

   #184779

   Purpose

   Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Activating internal tandem duplication (ITD) adde…

   Promoter

   MSCV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6TEV-UbG76C

   Plasmid

   #75299

   Purpose

   Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   human Ubiquitin mutant G76 to C
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Glycine 76 to Cystine

   Promoter

   T7

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6b

   Plasmid

   #215820

   Purpose

   Express CBE6b (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI (cas9 )
   

   Expression

   Mammalian

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. iF53 PB-Zim3-dCas9-mScarlet-Hygro

   Plasmid

   #204726

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmVenus(L68V)-mTurquoise2

   Plasmid

   #60493

   Purpose

   Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   SYFP2-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-alpha-synuclein-BiFC

    Plasmid

    #87855

    Purpose

    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    alpha-synuclein-VC155
    alpha-synuclein-VN154
    

    Tags

    C-terminal fragment of mVenus BiFC protein and N-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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