We narrowed to 1,186 results for: MS2;

1. phage - ubc - nls - 2xmcp - mCherry - BirA*

   Plasmid

   #131136

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-mCherry-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154787

   Purpose

   Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Fly, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and stdMCP

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

   Plasmid

   #131133

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-EGFP-cMycBirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA, cMyc (N-terminal of BirA*)

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-tdMCP-YFP

   Plasmid

   #99151

   Purpose

   Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   Dimeric MS2-coat binding protein
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHMM

   Plasmid

   #67717

   Purpose

   Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

   Depositor

   Robert Batey, Jeffrey Kieft

   Article

   Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

   Insert

   Hexahistidine/Maltose binding protein/MS2 coat protein fusion
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA polymerase

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AdML-M3

   Plasmid

   #11244

   Depositor

   Robin Reed

   Article

   Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

   Insert

   AdML-M3
   

   Use

   Tags

   3 MS2 hairpins

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

8. Ftz-M3

   Plasmid

   #11245

   Depositor

   Robin Reed

   Article

   Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

   Insert

   Ftz-M3
   

   Use

   Tags

   3 MS2 hairpins

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pWL078 MCP-2xGFP

   Plasmid

   #197047

   Purpose

   pCYC-NLS-MCP-2xGFP on pRS305

   Depositor

   Robert Singer

   Article

   Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

   Insert

   MCP-2xGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP561

    Plasmid

    #139497

    Purpose

    Expression vector for the MS2-GFP protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pADH1 MS2-GFPenvy tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pADH1

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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