We narrowed to 161,982 results for: addgene

1. pERF.1.0-gDNA

   Plasmid

   #112395

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF

   Depositor

   Kevin White

   Insert

   ERF (ERF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBCL6.1.0-gDNA

   Plasmid

   #112485

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6

   Depositor

   Kevin White

   Insert

   BCL6 (BCL6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYUB28b-MCS-optimized

   Plasmid

   #89179

   Purpose

   mycobacteria shuttle vector - pYUB28b with a single change to the MCS

   Depositor

   Ghader Bashiri, Ted Baker

   Article

   Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

   Type

   Empty backbone

   Use

   Mycobacteria shuttle vector

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG426Gal-GFP

   Plasmid

   #38310

   Depositor

   Susan Lindquist

   Article

   McLellan et al ACS Chem Biol. 2012 Jun 22.

   Insert

   EGFP (eGFP )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

5. 6OGK

   Plasmid

   #162259

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   MECP2 (MECP2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. MCTS1

   Plasmid

   #32049

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MCTS1 (MCTS1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E137A, K139A, Q140A

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pE2F3.3.1-gDNA

   Plasmid

   #113776

   Purpose

   CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3

   Depositor

   Kevin White

   Insert

   E2F3 (E2F3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 5T1I (CBX3)

   Plasmid

   #101885

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX3 (CBX3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. 5THA (Gemin5)

   Plasmid

   #101890

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Gemin5 (GEMIN5 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis, Thrombin cleavage

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. 4YZ8

    Plasmid

    #69955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. 5W9S (CXXC5)

    Plasmid

    #101266

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CXXC5 (CXXC5 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

    Plasmid

    #194723

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KNS2

    Plasmid

    #28122

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KNS2:HPC09H-C05:C207000 (KLC1 Human)
    

    Use

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pKG204

    Plasmid

    #63078

    Purpose

    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    V151I mutation compared to GenBank reference sequ…

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PHF20 (3QII)

    Plasmid

    #32039

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PHF20 (PHF20 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Tudor domain 2 only (amino acids 83-150)

    Promoter

    T7

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. HLTF

    Plasmid

    #28152

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    helicase-like transcription factor (HLTF Human)
    

    Use

    Tags

    thrombin cleavage

    Expression

    Bacterial

    Mutation

    Contains HIRAN domain (residues 51 to 171).

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

17. 6C1Y

    Plasmid

    #127292

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Insert

    mecp2 (MECP2 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 26, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. DDI1

    Plasmid

    #32867

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DDI1 (DDI1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 239-367 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. 5C7M (ITCH)

    Plasmid

    #69966

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH:SDC094-D06:C29524 (ITCH Human)
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. RFXANK (3V3O)

    Plasmid

    #36905

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RFXANK (RFXANK Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    amino acid residues 90-260 (ANK repeats)

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits