Addgene

We narrowed to 28,348 results for: REP

Showing: 1951 - 1980 of 28348 results

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pDRF1-GW sGFP2-T2A-mCherry

Plasmid

#118435

Purpose

Produces equimolar expression of sGFP2 and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

sGFP2-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW sYFP2-T2A-mTurquoise2

Plasmid

#118436

Purpose

Produces equimolar expression of sYFP2 and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

sYFP2-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW tagRFPT-T2A-mTurquoise2

Plasmid

#118438

Purpose

Produces equimolar expression of tagRFPT and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tagRFPT-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mCherry-T2A-mTurquoise2

Plasmid

#118423

Purpose

Produces equimolar expression of mCherry and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mCherry-T2A-mTq2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW Citrine-T2A-mTurquoise2

Plasmid

#118424

Purpose

Produces equimolar expression of Citrine and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

Citrine-T2A-mTq2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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KFP13

Plasmid

#119795

Purpose

RpoD5 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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KFP06

Plasmid

#119799

Purpose

RpoD6 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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KFP05

Plasmid

#119800

Purpose

RpoD5 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pVKG1-BSD

Plasmid

#108680

Purpose

Blasticidin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

SV40pro-BSD-polyA

Use

Tags

Expression

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA1

Plasmid

#107076

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA3

Plasmid

#107078

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA3

Plasmid

#107075

Purpose

M. sedula PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA2

Plasmid

#107077

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA2

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA2

Plasmid

#107074

Purpose

M. sedula PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA1

Plasmid

#107073

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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TM SE T206A

Plasmid

#79664

Purpose

More dimeric SE attached to Triple Mutant GEVI from Piao et al 2015

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Triple Mutant SE T206A

Use

Tags

Expression

Mammalian

Mutation

T206A

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAF1

Plasmid

#51425

Purpose

Artificial transcription factor with no activator domain

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pNH-NanoLuc

Plasmid

#173075

Purpose

Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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PARG-c002

Plasmid

#173107

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARG (PARG Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

S448-T976

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymNeongreen-T2A-mCherry

Plasmid

#125700

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymScarletI-T2A-mTurquoise2

Plasmid

#118441

Purpose

Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Plasmid

#52985

Purpose

split Venus attached to MCP and PCP respectively

Depositor

Robert Singer

Article

Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

Insert

nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

ubc

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pRN11

Plasmid

#84455

Purpose

shuttle plasmid for sarA P1-mCherry expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mCherry

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pPSU10-30-50-100

Plasmid

#173838

Purpose

Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p426MET25_sfpHluorin (MRV55)

Plasmid

#115697

Purpose

superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

Depositor

Eckhard Boles

Article

Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

Insert

sfpHluorin

Use

Tags

Expression

Yeast

Mutation

for introduced mutations see publication

Promoter

MET25

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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