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Showing: 3251 - 3300 of 9723 results

  5. pPRISM-QF2-he1.1:YFP

    Plasmid
    #184819
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    QF2-pA-he1.1:YFP
    Use
    CRISPR; Backbone for cloning of homology arms (fo…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184819-plasmid-map-sequence-id-366121

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  6. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184820-plasmid-map-sequence-id-366119

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  11. SUMO CRYGS 16-177 Q16C

    Plasmid
    #217673
    Purpose
    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine, and amino acids…
    Promoter
    T7
    Available Since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217673-plasmid-map-sequence-id-433254

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  28. pYG205

    Plasmid
    #200836
    Purpose
    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    P-BAD
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200836-plasmid-map-sequence-id-404931

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  46. pACT2-μ1A

    Plasmid
    #198168
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1A
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198168-plasmid-map-sequence-id-387525

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  47. pOP004

    Plasmid
    #185985
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Expression
    Bacterial
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185985-plasmid-map-sequence-id-378299

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  48. pOP005

    Plasmid
    #186030
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Expression
    Bacterial
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186030-plasmid-map-sequence-id-371540

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Showing: 3251 - 3300 of 9723 results