We narrowed to 8,805 results for: App

1. pJK514

   Plasmid

   #71577

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamine-435 to alanine

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK512

   Plasmid

   #71575

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes arginine-228 to glutamate

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS1122

   Plasmid

   #63790

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eSapphire-Twin-Strep tag followed by URA3
   

   Use

   E. coli cloning vector

   Tags

   eSapphire-Twin-Strep tag

   Promoter

   none

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. betaTrCP mVenus reporter

   Plasmid

   #192458

   Purpose

   Fluorescent reporter for beta-TrCP activity in human cells

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CDC25B (CDC25B Human)
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338

   Promoter

   EF1alpha

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEMS-Halo7Tag-hFis

   Plasmid

   #111136

   Purpose

   hFis with selflabeling tag, e.g. for fluorescence microscopy

   Depositor

   Karin Busch

   Article

   Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

   Insert

   human fission factor1 (FIS1 Human)
   

   Tags

   Halo7-tag

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRB133

   Plasmid

   #180176

   Purpose

   GluN2A in pCDNA3.1

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN2A (Grin2a Rat)
   

   Expression

   Mammalian

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEMS-Tom20-Halo7Tag

   Plasmid

   #111135

   Purpose

   Tom20 with selflabeling tag, e.g. for fluorescence microscopy

   Depositor

   Karin Busch

   Article

   Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

   Insert

   Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
   

   Tags

   Halo7 Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C2-Cep123-C2 domain

    Plasmid

    #73329

    Purpose

    Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

    Insert

    Cep123 (CEP89 Human)
    

    Tags

    EGFP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. HaloTag-WDR5

    Plasmid

    #200880

    Purpose

    Mammalian expression of human WDR5 with N-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HaloTag-WDR5 (WDR5 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eDrEGFR

    Plasmid

    #184000

    Purpose

    Optically controlled eDrEGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrEGFR (EGFR Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124542

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. eDrcKIT

    Plasmid

    #184001

    Purpose

    Optically controlled eDrcKIT expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrcKIT (KIT Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX_hTAF4_Cterminus

    Plasmid

    #44335

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF4 (TAF4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Functional C-terminal fragment 485-1085(end)

    Promoter

    LTR

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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