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pRDA_763

Plasmid

#201156

Purpose

EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRa

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

5x-ALFA tag-dEnAsCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

D908A

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL FLAG103QDProGFPp303

Plasmid

#15587

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pVAX1/mTyr-Cre

Plasmid

#99249

Purpose

Expresses Cre recombinase under tyrosinase promoter

Depositor

Christian Blank

Article

Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

Inserts

Cre recombinase
mTyr enh/prom

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL FLAG25QDProGFP p306

Plasmid

#15831

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL 103Q+ProGFPp416

Plasmid

#15583

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt103Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAGK112

Plasmid

#228127

Purpose

Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK111

Plasmid

#228126

Purpose

Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG for RT, U6/H1 for ncRNA/gRNA

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIV270

Plasmid

#226181

Purpose

Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TEF1p (Komagataella phaffii)

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT-ER

Plasmid

#8355

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

mouse estrogen receptor (G525R) on C-term

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-FOXO3a WT delta CT

Plasmid

#1796

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CT (aa468-673)

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
HA-FOXO3a WT DBM (H212R)

Plasmid

#8352

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

H212R

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA (pcDNA3-FLAG)

Plasmid

#35062

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

Contains HinA domain: aa 192-393

Promoter

CMV

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(515-710)-HinB (pcDNA3-FLAG)

Plasmid

#35063

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(515-710)-HinB (IFI16 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains HinB domain: aa 515-710

Promoter

CMV

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
GAL FLAG25QDProCFPp303

Plasmid

#15584

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Use

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG46QDProGFP p306

Plasmid

#15832

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL 46Q+ProGFPp416

Plasmid

#15581

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL 25Q+ProGFPp416

Plasmid

#15580

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pTZ tRNA_gRNA empty scaffold

Plasmid

#188450

Purpose

Expression of gRNAs using a human tRNA promoter

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

tRNA glutamine

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL 72Q+ProGFPp416

Plasmid

#15582

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q + Pro (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only