We narrowed to 6,084 results for: nls

1. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER20-Flag-NLS-hSPDEF delta1

   Plasmid

   #100874

   Purpose

   Lentiviral expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 1-248aa

   Promoter

   TRE2

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPL10p::pCGTAG (SPL10p::NLS-GFP)

   Plasmid

   #27410

   Depositor

   David Bartel

   Article

   Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

   Insert

   SPL10 promoter (AT1G27370 Mustard Weed)
   

   Use

   Tags

   Expression

   Mutation

   SPL10 promoter transcriptionally fused to a nucle…

   Promoter

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

   Plasmid

   #113437

   Purpose

   Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK 3XFLAG-MKK7-EE-NLS hygro

   Plasmid

   #87780

   Purpose

   Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK7 (Map2k7 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

6. enDR3-TEV-mEGFP-NLS (pRS1451)

   Plasmid

   #240546

   Purpose

   Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.

   Depositor

   Roman Szczęsny

   Article

   Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

   Insert

   enDR3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L52M

   Promoter

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. nb-enDR3-TEV-mEGFP-NLS (pRS1458)

   Plasmid

   #240547

   Purpose

   Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.

   Depositor

   Roman Szczęsny

   Article

   Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

   Insert

   nb-enDR3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S48A, K50A

   Promoter

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

   Plasmid

   #240455

   Purpose

   SARS-CoV-2 Protease 3CLpro expression plasmid

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Insert

   3CLpro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hUbCp

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-EFS-MCP-3XBFP-2xNLS

   Plasmid

   #236371

   Purpose

   Overexpression of MCP-3XBFP-2xNLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   MCP-3XBFP-2xnls
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-EFS-MCP-3XBFP-NLS

    Plasmid

    #236372

    Purpose

    Overexpression of MCP-3XBFPnls in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    MCP-3XBFPnls
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EFS-Halo-NLS-tDeg

    Plasmid

    #236370

    Purpose

    Overexpression of Halo-NLS-tDeg in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    Halo-NLS-tDeg
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231345

    Purpose

    Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

    Plasmid

    #232722

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within evoAPOBEC1: H47E+S48A; within Cas9 D10A

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGEN-Dre-nls (JDW 1131)

    Plasmid

    #229809

    Purpose

    A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    DRE recombinase-nls
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid

    #221540

    Purpose

    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

    Plasmid

    #222861

    Purpose

    This plasmid codes for the OgeuIscB nickase

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB Nickase
    

    Use

    CRISPR

    Tags

    3X HA and Nucleoplasmin NLS and ITR2

    Expression

    Mammalian

    Mutation

    E193A for nickase mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-fDIO-ArgiNLS-AausFP1

    Plasmid

    #220605

    Purpose

    Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-fDIO-ArgiNLS-mKate2

    Plasmid

    #220607

    Purpose

    Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-fDIO-ArgiNLS-oScarlet

    Plasmid

    #220606

    Purpose

    Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    oScarlet
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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