We narrowed to 1,479 results for: pbluescript

1. Met133ILeu, Met186ILeu ELL2

   Plasmid

   #127264

   Purpose

   For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Two Mets (133,186 )to ILeu Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Met331Leu ELL2

   Plasmid

   #127262

   Purpose

   For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met331 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met331 to ILeu Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Met1ILeu ELL2

   Plasmid

   #127258

   Purpose

   For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met1 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met1 to Ileu by Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-Cx43-FS

   Plasmid

   #65440

   Purpose

   Cloning, T7 RNA expression

   Depositor

   Trond Aasen

   Article

   Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

   Insert

   Cx43 (GJA1 Human)
   

   Use

   T7 in vitro rna production

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift (FS) mutation 35delA

   Promoter

   T7

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBNde(min)

   Plasmid

   #15125

   Depositor

   Franco Pagani

   Article

   Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

   Insert

   alpha-globin EDB fibronectin minigene (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   alpha-globin

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_PCP-T5Exo

   Plasmid

   #214736

   Purpose

   encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_PCP-T5Exo_bGH
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-P-CAG-GFPluc2

   Plasmid

   #80493

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   GFPluc2
   

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. fabp10a:CreERT2; cryaa:mCerulean

   Plasmid

   #230044

   Purpose

   Inducible Cre expression in Zebrafish hepatocytes

   Depositor

   Sumeet Pal Singh

   Article

   Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

   Insert

   CreERT2
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available since

   Feb. 11, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. WWP-Luc (p21/WAF1 promoter)

    Plasmid

    #16451

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHes7-Achilles-Hes7

    Plasmid

    #153528

    Purpose

    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

    Depositor

    Ryoichiro Kageyama

    Article

    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

    Insert

    Hes7 (Hes7 Mouse)
    

    Use

    Tags

    Achilles

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hes7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Marta Gallego, Lothar Hennighausen

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lox-stop-Lox p53 R172H

    Plasmid

    #14854

    Depositor

    Tyler Jacks

    Article

    Olive et al Cell. 2004 Dec 17. 119(6):847-60.

    Insert

    p53 R172H (Trp53 Mouse)
    

    Use

    Cre/Lox

    Tags

    Lox-STOP-lox

    Expression

    Mammalian

    Mutation

    Structural mutant R172H. (Murine p53 codon 172 co…

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTN4

    Plasmid

    #174212

    Purpose

    Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Inserts

    AtTIR1(F79G)::mRuby (TIR1 Nematode)
    GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    mRuby

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-AMVpl-β1WT-nAchR

    Plasmid

    #97080

    Purpose

    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSK+ chickBMPR-1B DN

    Plasmid

    #49529

    Purpose

    cloning vector for dominant negative chick BMP receptor 1B

    Depositor

    Lee Niswander, Peter ten Dijke

    Article

    Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

    Insert

    BMPR-1B DN (BMPR1B Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    dominant negative mutation (K231R); also P2A, M11…

    Promoter

    T3

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

19. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid

    #184397

    Purpose

    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin-PTC39
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    PTC in beta-globin at amino acid position 39

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36880

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    insulator
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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