160,990 results

1. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

   Plasmid

   #215769

   Purpose

   encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

   Depositor

   Dimitrios Wagner

   Article

   Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

   Inserts

   TRAC-5' homology arm (TRAC Human)
   P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
   TRAC-3' homology arm (TRAC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cas12a PAM mutated

   Promoter

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA304

   Plasmid

   #216072

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUPD2-HR1bR181A R185A-C (B3-B4)

   Plasmid

   #211855

   Purpose

   CDS for mutant HR1b degron in domestication vector

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   R181A R185A

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

   Plasmid

   #211861

   Purpose

   Nuclear-localized E3-DART ligase in domestication vector.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF0406

   Plasmid

   #143023

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CTCFL (CTCFL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156982

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PTGFRN (PTGFRN Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR/zeo-MACROD2

   Plasmid

   #172573

   Purpose

   For gateway cloning of N-terminal tagged MACROD2 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.cTNT.iCre

   Plasmid

   #69916

   Purpose

   Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

   Depositor

   William Pu

   Article

   Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

   Insert

   improved cre (iCre) recombinase and tdTomato
   

   Use

   AAV

   Tags

   The iCre coding frame and tdTomato coding frame i…

   Expression

   Mutation

   Promoter

   Chicken cardiac troponin T

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-eGFP-P2A-dTAG-Ing3

   Plasmid

   #170754

   Purpose

   Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

   Depositor

   Kristian Helin

   Article

   Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

   Insert

   eGFP-P2A-2xHA-dTAG
   

   Use

   Mouse Targeting

   Tags

   eGFP-P2A-2xHA-dTAG

   Expression

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   none

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL2Cas9

    Plasmid

    #98841

    Purpose

    Genome engineering of virulent phages

    Depositor

    Sylvain Moineau

    Article

    Lemay et al ACS Synth Biol. 2017 Mar 30. doi: 10.102

    Insert

    tracr/Cas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge