We narrowed to 6,010 results for: pcDNA 3.1

1. pcDNA3.1(+)-FluoSTEP-ICUE

   Plasmid

   #181845

   Purpose

   Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rat NCX1.1 (pcDNA3.1+)

   Plasmid

   #75190

   Purpose

   Mammalian expression of SLC8A1

   Depositor

   Jonathan Lytton

   Article

   Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

   Insert

   SLC8A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Pur-mEGFP

   Plasmid

   #186329

   Purpose

   Fluorescent reporter system

   Depositor

   Bertrand Collet

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-VEGFR2-mCherry

   Plasmid

   #139606

   Purpose

   Expresses a VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

   Plasmid

   #159129

   Purpose

   expresses NRF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+FLAG-KrascomG12D

   Plasmid

   #206844

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-2xFLAG-SREBP-2

   Plasmid

   #26807

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   L7V, G76S, K395R and I482L

   Promoter

   CMV

   Available since

   July 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-FGFR2c-V5/HIS

    Plasmid

    #201107

    Purpose

    expression of human FGFR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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