2,294 results

1. pCI-neo-myc-LC3(deltaC22, G120A)

   Plasmid

   #45449

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Encodes amino acids 1-120, Glycine at position 12…

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

2. LXSN-Cx43-S279/282A

   Plasmid

   #60713

   Purpose

   retroviral expression of rat Cx43 S279/282A mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S279/282A

   Promoter

   LTR

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. LXSN-Cx43-S279/282D

   Plasmid

   #60715

   Purpose

   retroviral expression of rat Cx43 S279/282D mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S279/282D

   Promoter

   LTR

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-dCpf1-BE0

   Plasmid

   #107678

   Purpose

   Expresses dLbCpf1-BE0 in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0
   

   Expression

   Mammalian

   Mutation

   D832A/E925/D1148A

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac HT JS-Munc18b

   Plasmid

   #135554

   Purpose

   Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18b (Stxbp2 Rat)
   

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDC183

   Plasmid

   #106415

   Purpose

   Co-expresses trans SNARE complex with pQZ210 in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Inserts

   SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
   SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
   

   Expression

   Bacterial

   Mutation

   Fragment 141-204, Codon optimization and Fragment…

   Promoter

   T7

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

   Plasmid

   #135565

   Purpose

   An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   miR-30 rat SST
   Nuc-EYFP
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   SYN1 and hSYN1

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dronpa-PSD95-N-14

   Plasmid

   #57293

   Purpose

   Localization: Membrane Scaffold, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Insert

   PSD95
   

   Tags

   Dronpa

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pGP-CMV-GCaMP3-44

   Plasmid

   #91780

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP3-44
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV GSE T329A Hygro (w189-4)

    Plasmid

    #22255

    Depositor

    Eric Campeau

    Insert

    Genetic suppressor element #22, T329A
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Threonine 329 is mutated to Alanine (p53 nomencat…

    Available Since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSM1-1-1

    Plasmid

    #15995

    Depositor

    Morris Birnbaum

    Article

    Birnbaum et al Cell. 1989 Apr 21. 57(2):305-15.

    Insert

    GLUT4 (Slc2a4 Rat)
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

12. PA-mCherry-miniSOG-Cx43-7

    Plasmid

    #57787

    Purpose

    Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Cx43
    

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

    Plasmid

    #32456

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S129A, S133A, S137A (S3V, G96R, and P108L also pr…

    Promoter

    SV40

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3.1 AT1AR-TSTS/4A

    Plasmid

    #45634

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-TSTS/A (Agtr1a Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Thr332, Ser335, Thr336, and Ser338 all changed to…

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

15. mEmerald-RatLC3B-C-7

    Plasmid

    #54147

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

    Insert

    RatLC3B
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pcDNA3.1/HisC 14-3-3 epsilon K49E

    Plasmid

    #11943

    Depositor

    Michael Yaffe

    Article

    Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

    Insert

    Ywhae (Ywhae Rat)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    K49E

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

17. prNFL-Q333P-EGFP

    Plasmid

    #132602

    Purpose

    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    Nefl (Nefl Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    998A>C, 999G>C; Gln333Pro

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Syn2ApcDNA4/TO

    Plasmid

    #14817

    Depositor

    Thomas Weimbs

    Article

    Low et al Dev Cell. 2003 May . 4(5):753-9.

    Insert

    Syntaxin2A (Stx2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PKC beta I dNPS

    Plasmid

    #16379

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta 1 (Prkcb Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: aa 30…

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only