-
Plasmid#139077PurposeLow phosphate inducible promoter pstSp for GFP expressionDepositorInsertpstSp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-cimA3.7
Plasmid#134595PurposeLow Phosphate inducible mutant citramalate synthaseDepositorInsertcimA3.7
UseTagsExpressionBacterialMutationI47V, E114V, H126Q, T204A, L238SPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-SBS-mCherry
Plasmid#134598PurposeLow Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)DepositorInsertSBS-mCherry
UseTagsmCherryExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
2ERT2-AAVS1 TALEN-L
Plasmid#120544PurposeDrug inducible TALEN targeting the human AAVS1 locus for genome editingDepositorInsertERT2, hAAVS1 1L TALEN
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK039
Plasmid#105425PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK038
Plasmid#105426PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI039
Plasmid#105428PurposeSynthetic biologyDepositorInsertLexA-VP16-ER (transcription factor: inducible expression; estradiol)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG643
Plasmid#105411PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI037
Plasmid#105413PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor) fused to mEGFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI041
Plasmid#105414PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG638
Plasmid#105389PurposeSynthetic biologyDepositorInsertpromotor CaBs3 (1000bp), variant
UseSynthetic BiologyTagsExpressionMutationTALE-binding site; BsaI/ BpiI restriction sites r…PromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG639
Plasmid#105390PurposeSynthetic biologyDepositorInsertpromotor CaBs3 (1000bp), variant
UseSynthetic BiologyTagsExpressionMutationTALE-binding site; BsaI/ BpiI restriction sites r…PromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_110μ-pcDNA3
Plasmid#111929PurposeCyan color bright luminescent calcium indicator with low affinityDepositorInsertCeNL(Ca2+)_110μ
UseTagsExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PfSSB_W166C
Plasmid#103001PurposeThe expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteineDepositorInsertPfSSB_W166C
UseTagsExpressionBacterialMutationC93A, W166CPromoterT7Available sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS345
Plasmid#89349PurposeBeetle Luciferase C-term tagging vectorDepositorInsertBeetle luciferase
UseC-terminal tagging vectorTagsExpressionMutationPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS346
Plasmid#89350PurposeRenilla Luciferase C-term tagging vectorDepositorInsertRenilla luciferase
UseC-terminal tagging vectorTagsExpressionMutationPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-rev
Plasmid#81215PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-for2
Plasmid#81209Purposefor targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iR
Plasmid#83314PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iR
UseTags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iR betwe…PromoterT5Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA5
Plasmid#83867PurposeGateway donor vector for IAA5DepositorInsertNP_173011.1 (IAA5 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF2)
Plasmid#61930PurposeExpression of hUCH37 (ISF2) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS2
Plasmid#65668PurposeMYC expression vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KQ
Plasmid#64982PurposeExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108QPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q HDEL
Plasmid#32124DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A GFP
Plasmid#32126DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A HDEL
Plasmid#32122DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-VHL(1-155)
Plasmid#29500DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsExpressionInsectMutationVHL(1-155)onlyPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 L94A-YFP
Plasmid#18692DepositorInsertRnq1 L94A (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys
Plasmid#15602DepositorInsertSup35 NM (SUP35 Budding Yeast, Candida albicans)
UseTags7xHis and CysteineExpressionBacterialMutation4 G/A. Four glycines mutated to alanines. The pos…PromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 E7K HA
Plasmid#8955DepositorInsertcyclin D2 (CCND2 Human)
UseTagsHAExpressionMammalianMutationE7K: point mutation in LxCxE motif, LxCxkPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJCNM(del22-68)
Plasmid#1095DepositorInsertSUP35 (SUP35 Budding Yeast)
UseTagsHisExpressionBacterialMutationamino acids 20 - 68 DELETED from NM domain of SUP…PromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pG1-NMGR526(d22-69)
Plasmid#1125DepositorUseTagsGR526ExpressionYeastMutationnucleotides encoding NM domain amino acids 22-69 …PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJLI-Sup35N-KDG6-C
Plasmid#1238DepositorInsertSUP35 (SUP35 Budding Yeast)
UseYeast integrative plasmidTagsExpressionMutationSUP35 middle region (aa124-253) was replaced with…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLN432 (FuGW-G14p-mKate2)
Plasmid#105187PurposeModule 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2DepositorInsertG14p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B R398,399Q IRES GFP
Plasmid#133819PurposeEncodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationR398,399QPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B K326,327E IRES GFP
Plasmid#133820PurposeEncodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationK326,327EPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
UseTagsExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2-NOS2Promoter-Luciferase
Plasmid#19296DepositorInsertNOS2 Promoter (Nos2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1wt
Plasmid#131810PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-CDC25A
Plasmid#216511PurposeExpresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoterDepositorInsertCDC25A (CDC25A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin Helper
Plasmid#127694PurposePlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Glycoproteins
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP shRNA tet pLKO puro
Plasmid#163017PurposeControl tet-inducible shRNA targeting eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only