We narrowed to 2,341 results for: PUC19

1. qTAG-N-Puro-miRFP670nano3

   Plasmid

   #207691

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT9675-CBE

   Plasmid

   #163007

   Purpose

   plasmid expressing CBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   CBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Zeo-mScarlet

   Plasmid

   #207700

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pHBJT001

   Plasmid

   #225152

   Purpose

   High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-mNeon-Zeo

   Plasmid

   #207731

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-C-miRFP670nano3-Puro

   Plasmid

   #207724

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. qTAG-N-Zeo-mNeon

   Plasmid

   #207698

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. qTAG-N-Puro-V5

    Plasmid

    #207697

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-N-Zeo-V5

    Plasmid

    #207708

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Aurli_150841_GZG

    Plasmid

    #162563

    Purpose

    Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

    Depositor

    Jackie Collier

    Article

    Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

    Insert

    shble
    

    Use

    Unspecified

    Promoter

    GAPDH

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Expression

    Bacterial

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-moxGFP-Blast

    Plasmid

    #207710

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLinker-AID-3xHA-DHFR-LoxP

    Plasmid

    #86669

    Purpose

    for generation of AID strains using CRISPR tagging technology

    Depositor

    David Sibley

    Article

    Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

    Insert

    Linker-AID-3xHA-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1α-Cre-2A-miRFP670nano3

    Plasmid

    #207747

    Purpose

    Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-miRFP670nano3
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-3xHA-Zeo

    Plasmid

    #207740

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. qTAG-N-Zeo-3xHA

    Plasmid

    #207707

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge