We narrowed to 2,128 results for: Pam

1. pcDNA3-FLAG-MTOR-I2500F

   Plasmid

   #69014

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-I2500F (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Isoleucine 2500 to Phenylalanine

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-MTOR-E1799K

   Plasmid

   #69009

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-E1799K (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Glutamate 1799 to Lysine

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

   Plasmid

   #139987

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-dLight1.2

   Plasmid

   #111068

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1 and AAV5

   Insert

   dLight1.2
   

   Use

   AAV

   Tags

   Flag tag

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

   Plasmid

   #184632

   Purpose

   Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad67 (Gad1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pTH-iCre:EGFP-WPREpA

   Plasmid

   #213142

   Purpose

   Truncated rat TH promoter expressing iCre fused to EGFP

   Depositor

   Ulrik Gether

   Article

   Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

   Insert

   iCre
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Truncated TH promoter from rat (Rattus norvegicus…

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-SPOTlight-U2GCR

   Plasmid

   #164819

   Purpose

   Reporter to measure ISR state-dependent protein synthesis

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   SPOTlight-U2GCR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-MTOR-L1460P

   Plasmid

   #69006

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-L1460P (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Leucine 1460 to Proline

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PiggyBac-TetO-Ascl1-neomycin

   Plasmid

   #176482

   Purpose

   TetO-Ascl1 in a PiggyBac vector with neomycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV.PGK.mFoxa2

    Plasmid

    #33014

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H10L

    Available Since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-pTH-iCre-WPREpA

    Plasmid

    #213130

    Purpose

    Truncated rat TH promoter expressing iCre

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-dLight1.1

    Plasmid

    #111067

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1 and AAV5

    Insert

    dLight1.1
    

    Use

    AAV

    Tags

    Flag tag

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-FLAG-MTOR-C1483F

    Plasmid

    #69008

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-C1483F (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Cysteine 1483 to Phenylalanine

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

17. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

    Plasmid

    #191691

    Purpose

    For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hsyn-GRAB_rDA-mut

    Plasmid

    #140558

    Purpose

    Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-dLight1.1

    Plasmid

    #111066

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1

    Insert

    dLight1.1
    

    Use

    AAV

    Tags

    Flag tag

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only