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Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gCAG
Plasmid#114384PurposepPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAGDepositorInsertgCAG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR
Plasmid#60619PurposecrRNA expression plasmid on pRS423 backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTW072
Plasmid#190247PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW069
Plasmid#190246PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gDM1d
Plasmid#114387PurposepPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGGDepositorInsertgDM1d
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196084Purpose(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196080Purpose(Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Blasticidin S deaminase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH030
Plasmid#159633PurposeCRISPR‐Cas9 tool for the genetic engineering of Bacillus phagesDepositorTypeEmpty backboneUseCRISPR; Shuttle vectorTagsExpressionMutationPromoterConstitutive tracrRNAAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
phU1-CasRx-non-targeting (pKW82)
Plasmid#229050PurposeCloning backbone for CasRx crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-non-targeting (pJK5)
Plasmid#229052PurposeCloning backbone for PspCas13b crRNA (U6 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
phU1-PspCas13b-non-targeting (pEZ36)
Plasmid#229053PurposeCloning backbone for PspCas13b crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XMS2
Plasmid#75389PurposesgRNA1-2XMS2DepositorInsertsgRNA1-2XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmU1-CasRx-non-targeting (pKW83)
Plasmid#229051PurposeCloning backbone for CasRx crRNA (Mouse U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XPP7
Plasmid#75390PurposesgRNA1-2XPP7DepositorInsertsgRNA1-2XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XboxB
Plasmid#75391PurposesgRNA1-2XboxBDepositorInsertsgRNA1-2XboxB
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRGE31
Plasmid#50929PurposeExpressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 and dual 35S promoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only