We narrowed to 3,631 results for: WOL

1. pIG-730pUC19-tNGFR-P2A-Coronin1A

   Plasmid

   #186110

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CORONIN1A
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

   Plasmid

   #117405

   Purpose

   SpyCas9 sgRNA 1 targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SpyCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-p21_WT

   Plasmid

   #40202

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

   Plasmid

   #163979

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

   Plasmid

   #166791

   Purpose

   Mammalian cell expression vector for His-tagged DPP9S

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNH605-CCAAT-YFP

   Plasmid

   #172132

   Purpose

   Fluorescent reporter for HAP complex activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xCCAAT-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-P2A-CIAR_pLenti_CMV_Puro

   Plasmid

   #86503

   Purpose

   Lenti plasmid for generating CIAR expressing stable cell lines

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   GFP-P2A-CIAR (SOS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-mouse c-MycV394D-IRES-RFP

   Plasmid

   #73573

   Purpose

   Mammalian retroviral expression of mouse Myc V394D mutant

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (Myc Mouse)
   

   Use

   Retroviral

   Tags

   IRES-RFP

   Expression

   Mammalian

   Mutation

   V394D

   Promoter

   MSCV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

   Plasmid

   #117406

   Purpose

   SpyCas9 sgRNA 2 targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SpyCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 LIC 6A FLAG-TEV-DPP8

    Plasmid

    #166795

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac HTB DPP9L

    Plasmid

    #166788

    Purpose

    Insect cell expression vector for DPP9L (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac HTB DPP9S

    Plasmid

    #166787

    Purpose

    Insect cell expression vector for DPP9S (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 LIC 6A HIS-TEV-DPP8

    Plasmid

    #166793

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

    Plasmid

    #117411

    Purpose

    AsCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    AsCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac HTB DPP9S S730A

    Plasmid

    #169946

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    S730A

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mEmerald-Cx32-7

    Plasmid

    #54054

    Purpose

    Localization: Gap Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fort et al J Biol Chem. 2011 Jul 1;286(26):22875-85

    Insert

    Cx32 (GJB1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pDB.His.MBP.3C NLRP1 CARD WT

    Plasmid

    #164008

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid

    #140202

    Purpose

    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Tags

    Expression

    Mutation

    G174D

    Promoter

    PelcA

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac HTB DPP9S D808N

    Plasmid

    #169947

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    D808N

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Tags

    Expression

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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