We narrowed to 165,420 results for: addgene

1. pBabe H-Ras V12 G37

   Plasmid

   #18745

   Depositor

   Scott Lowe

   Insert

   H-Ras V12 C40 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V12 / G37

   Available Since

   July 7, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pKG113

   Plasmid

   #63073

   Purpose

   Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   CENP-T (CENPT Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Mutation

   E. coli codon optimized. G217R mutation compared …

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYUB28b-MBP

   Plasmid

   #89178

   Purpose

   mycobacteria shuttle vector

   Depositor

   Ted Baker, Ghader Bashiri

   Article

   Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

   Type

   Empty backbone

   Use

   Mycobacteria shuttle vector

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. RBBP4

   Plasmid

   #25350

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RBBP4-13 (RBBP4 Human)
   

   Use

   Baculovirus expression

   Tags

   His

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. 4RFQ

   Plasmid

   #64656

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   METTL18:PBC004-E03:C231318 (METTL18 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   May 15, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pERF.1.0-gDNA

   Plasmid

   #112395

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF

   Depositor

   Kevin White

   Insert

   ERF (ERF Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBCL6.1.0-gDNA

   Plasmid

   #112485

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6

   Depositor

   Kevin White

   Insert

   BCL6 (BCL6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYUB28b-MCS-optimized

   Plasmid

   #89179

   Purpose

   mycobacteria shuttle vector - pYUB28b with a single change to the MCS

   Depositor

   Ted Baker, Ghader Bashiri

   Article

   Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

   Type

   Empty backbone

   Use

   Mycobacteria shuttle vector

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426Gal-GFP

   Plasmid

   #38310

   Depositor

   Susan Lindquist

   Article

   McLellan et al ACS Chem Biol. 2012 Jun 22.

   Insert

   EGFP (eGFP )
   

   Expression

   Yeast

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

10. MCTS1

    Plasmid

    #32049

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MCTS1 (MCTS1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E137A, K139A, Q140A

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. 6OGK

    Plasmid

    #162259

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    MECP2 (MECP2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pE2F3.3.1-gDNA

    Plasmid

    #113776

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3

    Depositor

    Kevin White

    Insert

    E2F3 (E2F3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5T1I (CBX3)

    Plasmid

    #101885

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX3 (CBX3 Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. 5THA (Gemin5)

    Plasmid

    #101890

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Gemin5 (GEMIN5 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis, Thrombin cleavage

    Promoter

    polyhedrin

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. 4YZ8

    Plasmid

    #69955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. 5W9S (CXXC5)

    Plasmid

    #101266

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CXXC5 (CXXC5 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. KNS2

    Plasmid

    #28122

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KNS2:HPC09H-C05:C207000 (KLC1 Human)
    

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Available Since

    March 22, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

18. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

    Plasmid

    #194723

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKG204

    Plasmid

    #63078

    Purpose

    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    V151I mutation compared to GenBank reference sequ…

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHF20 (3QII)

    Plasmid

    #32039

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PHF20 (PHF20 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Tudor domain 2 only (amino acids 83-150)

    Promoter

    T7

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits