We narrowed to 10,281 results for: aga
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Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only
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pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP
Plasmid#114730PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M7
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_110μ-pcDNA3
Plasmid#111929PurposeCyan color bright luminescent calcium indicator with low affinityDepositorInsertCeNL(Ca2+)_110μ
UseTagsExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G11-R0
Plasmid#101805PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SsoPCNA3
Plasmid#107069PurposeExpresses S. solfataricus PCNA3 fused to MBP in E. coliDepositorInsertS. solfataricus PCNA3
UseTags6xHis-thrombin cleavage site and MBPExpressionBacterialMutationPromotertac promoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTADA1.1580
Plasmid#105568Purposeretrovirally express TADA1 shRNA with puro resistance and GFP markerDepositorInsertTADA1 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF2.1635
Plasmid#105561Purposeretrovirally express TAF2 shRNA with puro resistance and GFP markerDepositorInsertTAF2 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G14-R0
Plasmid#101806PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R0
Plasmid#101804PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1R
Plasmid#86969PurposeExpresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDHPromoterT7 promoterAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-016
Plasmid#78704PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-015
Plasmid#78703PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-010
Plasmid#78697PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-008
Plasmid#78695PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-007
Plasmid#78694PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(1)
Plasmid#73550PurposeshRNA(1) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB BmOr2 (Y464A)
Plasmid#73927PurposeUAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to AlanineDepositorInsertBmOr2 (Y464A)
UseTagsExpressionInsectMutationchanged 464 Tyrosine to AlaninePromoterhsp70Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS4
Plasmid#69236PurposeU6 driven SpCas9 sgRNA expression for TS4 siteDepositorInsertTS4 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL671
Plasmid#62825PurposeLim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsglgC leaderExpressionBacterialMutationPromoterpConAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1017
Plasmid#62828PurposeLim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsm8::glgC leaderExpressionBacterialMutationPromoterpConNoHindAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only