We narrowed to 6,085 results for: pcDNA 3.1

1. pcDNA3.1 MCS-BirA(R118G)-HA

   Plasmid

   #36047

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

   Depositor

   Kyle Roux

   Article

   Roux et al J Cell Biol. 2012 Mar 12.

   Has Service

   Cloning Grade DNA

   Insert

   BirA(R118G)-HA
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Available Since

   April 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-dCas9-dMQ1-EGFP

   Plasmid

   #89637

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+ eiF4A1 myc HIS

   Plasmid

   #71657

   Purpose

   mammalian expression of eiF4A1

   Depositor

   Gideon Dreyfuss

   Insert

   eiF4A1 (EIF4A1 Human)
   

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   aa403 from Ala to Arg

   Promoter

   CMV

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+-mutTornado-split-nLuc

   Plasmid

   #212707

   Purpose

   Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   mutTornado-CVB3-split-nLuc
   

   Mutation

   Deleted the partial 3'ribozyme sequence in T…

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Flag-PGC-1alpha1

   Plasmid

   #45501

   Depositor

   Bruce Spiegelman, Jorge Ruas

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform1 (Ppargc1a Mouse)
   

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Q339K relative to NM_008904

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-FLT3-D835Y-V5/HIS

   Plasmid

   #236007

   Purpose

   expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   D835Y substitution

   Promoter

   CMV

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) Flag-His-ATM kd

   Plasmid

   #31986

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM kd (ATM Human)
   

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   3'UTRf 9 nucl/D2870A N2875k. catalytically …

   Available Since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-SARS-CoV-2-S (Wuhan)

    Plasmid

    #215812

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MS2-HA-HsStaufen2-T1_V

    Plasmid

    #147741

    Purpose

    Mammalian Expression of HsStaufen2-T1

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen2-T1 (STAU2 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-MS2CP-VPg(FCV)

    Plasmid

    #167314

    Purpose

    Template pDNA to prepare CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-2xFLAG-2xSTREP-CCND2

    Plasmid

    #172629

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 VDAC1-Linker-GFP

    Plasmid

    #211734

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-ZNF598-TEV-3xFLAG

    Plasmid

    #105690

    Purpose

    Expresses C terminally 3xFLAG tagged ZNF598 protein

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Insert

    ZNF598 (ZNF598 Human)
    

    Tags

    TEV-3xFLAG

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/Puro-CAG-JEDI-1P

    Plasmid

    #202607

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HNF1B-WT-Flag-UTR

    Plasmid

    #183239

    Purpose

    Expression of FLAG tagged HNF1B

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 ZoAl-ENT N-1F (R1103A)

    Plasmid

    #216548

    Purpose

    For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-TFEBS211A-MYC (#805)

    Plasmid

    #99957

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

    Insert

    TFEB (TFEB Human)
    

    Tags

    6xHIs and Myc

    Expression

    Mammalian

    Mutation

    S211A

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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