We narrowed to 2,306 results for: puc19

1. pILGFPQ4

   Plasmid

   #83559

   Purpose

   Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL7 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT15483_e-ABE8e

   Plasmid

   #174121

   Purpose

   Expresses ABE8e fused with eCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   eABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT15001-AncBE4max

   Plasmid

   #163016

   Purpose

   plasmid expressing AncBE4max

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   AncBE4max
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIF251

   Plasmid

   #133935

   Purpose

   Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.

   Depositor

   IIya Finkelstein

   Article

   Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

   Insert

   Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
   

   Use

   Λ-phage recombineering plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pVC_CRISPR_Cat

   Plasmid

   #120368

   Purpose

   Reporter plasmid for full-site selection assay

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   CRISPR_Cat
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVD_CRISPR

   Plasmid

   #120370

   Purpose

   Plasmid containing shortened version of V-D CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP1698-4

   Plasmid

   #105242

   Purpose

   mammalian eGFP

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP3

   Plasmid

   #65451

   Purpose

   pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Type

   Empty backbone

   Use

   Tags

   yEGFP reporter

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pITGFP3

   Plasmid

   #65452

   Purpose

   pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Type

   Empty backbone

   Use

   Tags

   yEGFP-CLN2PEST reporter

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

10. BC1364-mouse U6-sgH2B

    Plasmid

    #164043

    Purpose

    U6-driven sgRNA targeting H2B

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    H2B targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT9749-dABE

    Plasmid

    #162998

    Purpose

    plasmid expressing ABE with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT15002-ABEmax

    Plasmid

    #163006

    Purpose

    plasmid expressing ABEmax

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    ABEmax
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAT15064-eCBE

    Plasmid

    #163009

    Purpose

    plasmid expressing eCBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAT9676-ABE

    Plasmid

    #162997

    Purpose

    plasmid expressing ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML1

    Plasmid

    #108332

    Purpose

    MIDAS Level-1 source vector for cloning of modules (synthetic biology parts)

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAID8Z104

    Plasmid

    #165056

    Purpose

    Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pILGFPQ3

    Plasmid

    #83558

    Purpose

    Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL2

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDiySpike2

    Plasmid

    #136949

    Purpose

    Plasmid for the In Vitro transcription of DiySpike2

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAVS1-NEO-CAG-BFP-pA-GFP-pA

    Plasmid

    #149344

    Purpose

    Vector for targeting the Replace reporter to the AAVS1 locus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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