-
Plasmid#123235PurposeExpresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry
Plasmid#214470PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9-T2A-mCherry
Plasmid#135012PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
UseTags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutationPromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX
Plasmid#154910Purpose1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.DepositorInsertPhytochrome B (PHYB Mustard Weed, Synthetic)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYBPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry
Plasmid#172620PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)
Plasmid#86416PurposeONM/ER split GFP11 reporter with mCherryDepositorInsertNOP1pr-GFP11-mCherry-Scs2TM
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)
Plasmid#72639PurposeTol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domainDepositorInsertUAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
UseGatewayTagsExpressionMutationStop codon after mCherry- SEE DEPOSITOR COMMENTS …PromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR
Plasmid#200636PurposeStrong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
Plasmid#216531PurposeTargeting vector for CRISPRd generationDepositorInsertABI-dCas9-mCherry
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)
Plasmid#191141PurposePositive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codonDepositorInsertmCherry:s70587-W:EGFP
UseTagsExpressionMammalianMutationUAG>UGG (stop>Trp)PromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2
Plasmid#110058PurposePer2 transcription fluorescence reporterDepositorInsertPer2 promoter and mCherry and Venus (Per2 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianMutationPromoterPer2Available sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP-SFPQ-IRES-mCherry
Plasmid#166950PurposeLentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoterDepositorInsertSFPQ (SFPQ Human)
UseLentiviralTagsGFP and mCherryExpressionMutationPromotereF1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)
Plasmid#191169PurposeExpression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP outputDepositorInsertmCherry:p53(R248Q).s2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
Plasmid#35502PurposeAAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationC128S and D156APromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)
Plasmid#191144PurposeExpression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp longDepositorInsertmCherry:Bdnf.s1:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)
Plasmid#191180PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s3:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG FLEX BARK D110A p2A mCherry
Plasmid#117694PurposeCAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencingDepositorInsertBARKrgs D110A peptide
UseCre/LoxTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179117PurposeExpresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertFermt2 sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
Plasmid#135014PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
UseTags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the C-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry
Plasmid#172627PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry
Plasmid#135013PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
UseTags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the N-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
FH1-CLGT#2-mCherry-_PGK-H2B-CFP
Plasmid#164106PurposeFluorescent reporter for genetic tracing of classical Glioblastoma cell-state.DepositorInsertCLGT#2-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2-mCherry
Plasmid#137144PurposeIntersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationH134RPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)
Plasmid#86417Purposeluminal split GFP11 reporter with mCherryDepositorInsertNOP1pr-mCherry-SCS2TM-GFP11
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human NMyc-humanER-IRES-mCherry
Plasmid#73571PurposeMammalian retroviral expression of NMyc-ERDepositorInsertNMyc (MYCN Human)
UseRetroviralTagsHuman ER and IRES-mCherryExpressionMammalianMutationPromoterMSCVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChR2-mCherry
Plasmid#137142PurposeIntersectional viral expression of ChR2-mCherry in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationH134RPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)
Plasmid#189795PurposeExpress GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Mouse, Human)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4
Plasmid#125148PurposeLentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.DepositorInsertAcrIIA4-P2A-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV ArcLightCo (Q239)-T2A-nls-mCherry
Plasmid#85806PurposeGenetically encoded voltage sensor ArcLight- codon optimizedDepositorInsertArcLightCo-Q239
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC
Plasmid#162099PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
UseLentiviralTagsVSVg-StrepTagII-ProtCExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173874PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry
Plasmid#136603PurposeEncodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertFKBP-C-mSOG-J-alpha
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
Plasmid#139649PurposeReporter assayDepositorInsertH2B-mCherry
UseLentiviralTagsFLAG, His6ExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-p53 gRNA-Cas9-T2A-mCherry
Plasmid#164256PurposeTranscription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsg p53
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-uTEV2-V5; UAS-mCherry
Plasmid#173123PurposeExpresses uTEV2 and reporter receiver construct in DIPGDepositorInsertArrestin-uTEV2-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry-t2a-SOX2-p2a-KLF4
Plasmid#193296Purposeepisomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)DepositorUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA
Plasmid#154869PurposeHuman synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherryDepositorInsertrM3D(Gs)
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]
Plasmid#159900PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mcherry | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterPmlc-1Available sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-mCherry
Plasmid#137133PurposeIntersectional viral expression of mCherry in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2
Plasmid#194515PurposeP2 promoter for transgenic enhancer assays in zebrafishDepositorInsertEnhanced Green Fluorescent Protein
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113761PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-loxp hM4Di-mCherry
Plasmid#161581PurposeExpression of hM4Di-mCherry in a cre-dependent fashionDepositorInserthM4Di-mCherry
UseAAVTagsExpressionMutationNAPromoterhSynAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168170PurposeExpression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
UseTagsYFPExpressionWormMutationE497K and C566DPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-DIO-TRPV1-P2A-mCherry
Plasmid#200831PurposeIn the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherryDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+10A)-IDR-mCherry
Plasmid#145282PurposeBacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion ProteinDepositorInsertHOXD13(+10A)-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2-NLS
Plasmid#145260PurposeMammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLSDepositorInsertHOXD13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only