We narrowed to 2,365 results for: DAP

1. IDA3::NeonGreen N-term (WS14)

   Plasmid

   #126875

   Purpose

   NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.

   Depositor

   Winfield Sale

   Article

   Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

   Insert

   IDA3
   

   Use

   Other

   Tags

   NeonGreen

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. IDA3::NeonGreen exon2 (WS15)

   Plasmid

   #126876

   Purpose

   NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

   Depositor

   Winfield Sale

   Article

   Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

   Insert

   IDA3
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCML 313

   Plasmid

   #98586

   Purpose

   pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Insert

   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCML 314

   Plasmid

   #98584

   Purpose

   pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Inserts

   CsrA
   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Danio rerio cryaa V62T

   Plasmid

   #47630

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Changed Valine 62 to Threonine

   Promoter

   T7

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Danio rerio cryaa T147V

   Plasmid

   #47628

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Changed Theronine 147 to Valine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Danio rerio cryaa C143S

   Plasmid

   #47619

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 143 to Serine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. EC.SurA.pTYB1.a21-281&390-428.M231R

   Plasmid

   #15958

   Depositor

   David McKay

   Article

   Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

   Insert

   Escherichia coli surA delta PPIase domain 2
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   residues 282-389 deleted; M231R mutation

   Promoter

   Available Since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pX330-PITCh-TUBA1B

   Plasmid

   #207763

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-CETN2

    Plasmid

    #227291

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVtight-UPRT-T2A-RFP-IRES-CD

    Plasmid

    #126677

    Purpose

    Doxycycline inducible expression of UPRT and CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-PITCh-H3C2

    Plasmid

    #207780

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK5-FLAG-p14

    Plasmid

    #42330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p14 (LAMTOR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid

    #217319

    Purpose

    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

    Depositor

    Noboru Mizushima

    Article

    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

    Insert

    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    

    Use

    Retroviral

    Tags

    HaloTag and SNAP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmiRGLO Dual-Luciferase-MYB-3'UTR

    Plasmid

    #182250

    Purpose

    Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of MYB gene
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-TS2126 RNA Ligase

    Plasmid

    #119941

    Purpose

    Express TS2126 RNA ligase in E. coli

    Depositor

    Takashi Ito

    Article

    Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

    Insert

    TS2126 RNA ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid

    #227273

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-FLAG-FADD

    Plasmid

    #78802

    Purpose

    To overexpress FADD in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

    Insert

    FADD (FADD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP-1 mu1-GFP

    Plasmid

    #173432

    Purpose

    Expression of AP-1 Mu1 subunit with a terminal GFP

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP1M1 (Ap1m1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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