We narrowed to 2,422 results for: NIG

1. pCAG_GST-TEV-EGFP-ATG13

   Plasmid

   #223797

   Purpose

   Expression of recombinant protein for purification

   Depositor

   James Hurley, Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AN-DDK_WT POLI

   Plasmid

   #131228

   Purpose

   Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Coding sequence has been codon optimised for expr…

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-H2BmGreenLantern

   Plasmid

   #177332

   Purpose

   To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

   Depositor

   Pantelis Tsoulfas, Murray Blackmore, Gregory Petsko

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   H2B (Hist1h2bq Rat)
   

   Use

   AAV

   Tags

   mGreenLantern

   Expression

   Mammalian

   Mutation

   This fusion protein was optimized to the Human co…

   Promoter

   CAG

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xFNLS-P2A-Puro

   Plasmid

   #110872

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-IRES-blast GFP-SPNS1

   Plasmid

   #198571

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB1.HMBP.PrS.JARID2

   Plasmid

   #125166

   Purpose

   Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   JARID2 (JARID2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.dEZH2

   Plasmid

   #220241

   Purpose

   Expression of an EZH2 catalytic site mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   dEZH2 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   Y731F

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

    Plasmid

    #118274

    Purpose

    Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Inserts

    bPAC(WT)
    SthK
    

    Use

    AAV

    Tags

    His tag, P2A, mCherry, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CamK II

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIV-EGFP.Flag-EZH2-mt2*

    Plasmid

    #220246

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2* (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1.HMBP.PrS.EPOP

    Plasmid

    #125170

    Purpose

    Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EPOP (EPOP Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-FUW-Mecom 1

    Plasmid

    #118590

    Purpose

    Expresses Mecom isoform 1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Mecom (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pD2_miniluc_newP7

    Plasmid

    #174105

    Purpose

    adding the mini luciferase and mini promoter to the MPRA lenti construct

    Depositor

    Paul Khavari

    Article

    Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

    Insert

    mini luciferase mini-promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIG-hNFATc2/C - AVITEV

    Plasmid

    #74058

    Purpose

    retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-xBE3-P2A-Puro

    Plasmid

    #110870

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO-FUW-Mecom 2

    Plasmid

    #118591

    Purpose

    Expresses Mecom isoform 2 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Mecom (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid

    #191100

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    mScarlet-I-Cdt1 (30-120)
    

    Use

    AAV and Synthetic Biology

    Tags

    mScarlet-I fused to residues 30-120 of human Cdt1…

    Expression

    Mammalian

    Mutation

    Optimized to human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75236

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-hH4-AVITEV

    Plasmid

    #74051

    Purpose

    retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Histone-H4 (H4C16 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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