We narrowed to 1,419 results for: RCA

1. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS647

   Plasmid

   #183176

   Purpose

   PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

   Depositor

   Timothy Whitehead

   Article

   Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

   Insert

   PYR1 (PYR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SBC015866

   Plasmid

   #226280

   Purpose

   Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

   Depositor

   Rainer Breitling

   Article

   Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

   Inserts

   4'-phosphopantetheinyl transferase Sfp
   Cinnamyl alcohol dehydrogenase
   Carboxylic acid reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. FR_HNF1B

   Plasmid

   #31101

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUMO His6 SUMO PYL1 (36-211)

   Plasmid

   #177843

   Purpose

   Bacterial Expression of PYL1

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 1 (PYL1 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDicAID_nCas9-PmCDA-2A-NptII_ETR

   Plasmid

   #91695

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUDE722

   Plasmid

   #103022

   Purpose

   expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crHCAN1-4.S (CAN1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FR_HNF1B_A263insGG

   Plasmid

   #31103

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   mutation A263insGG

   Promoter

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. FR_HNF1B_P328L329del

   Plasmid

   #31102

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   mutation P328L329delCCTCT

   Promoter

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFETCh_ARID4B

    Plasmid

    #101072

    Purpose

    Donor vector for 3' FLAG tag of human ARID4B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ARID4B homology arms (ARID4B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/V5-His-mPer1

    Plasmid

    #219421

    Purpose

    Expresses 3873 bp of mPer1 coding sequence in E. coli.

    Depositor

    Aziz Sancar

    Article

    Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mPer1 (Per1 Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SBC015869

    Plasmid

    #226281

    Purpose

    Expresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

    Depositor

    Rainer Breitling

    Article

    Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

    Inserts

    4'-phosphopantetheinyl transferase Sfp
    Cinnamyl alcohol dehydrogenase
    Carboxylic acid reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET24a His6 GST PYL2(14-188)

    Plasmid

    #177842

    Purpose

    Bacterial Expression of PYL2

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 2 (PYL2 Mustard Weed)
    

    Use

    Tags

    6xHis-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT140_GalL_cCDA1-VRERBE3

    Plasmid

    #145103

    Purpose

    Expressing base editor cCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT122_GalL_cCDA1-VQRBE3

    Plasmid

    #145087

    Purpose

    Expressing base editor cCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_ARID4B_1

    Plasmid

    #101078

    Purpose

    Encodes gRNA for 3' target of human ARID4B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEL gRNA (BRDN0001146030)

    Plasmid

    #77053

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. RNASEL gRNA (BRDN0001148937)

    Plasmid

    #77054

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mcherry-DN KASH

    Plasmid

    #125553

    Purpose

    mcherry labeled DN-KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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