Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 63,651 results for: cloning

Clear keywords
Showing: 1341 - 1360 of 63651 results

  2. AcGFP1-N1

    Plasmid
    #54705
    Purpose
    Localization: N1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AcGFP1
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54705-plasmid-map-sequence-id-162699

    Enlarge

  4. pVITRO1-Trastuzumab-IgG3/κ

    Plasmid
    #61885
    Purpose
    Expresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61885-plasmid-map-sequence-id-404037

    Enlarge

  5. pVITRO1-Trastuzumab-IgM/κ

    Plasmid
    #61881
    Purpose
    Expresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Mu heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61881-plasmid-map-sequence-id-101188

    Enlarge

  7. mNeptune2-C1

    Plasmid
    #54836
    Purpose
    Localization: C1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54836-plasmid-map-sequence-id-132753

    Enlarge

  8. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118154-plasmid-map-sequence-id-239465

    Enlarge

  9. AcGFP1-C1

    Plasmid
    #54607
    Purpose
    Localization: C1 Cloning Vector, Excitation: 475, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AcGFP1
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54607-plasmid-map-sequence-id-162738

    Enlarge

  10. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid
    #86928
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and td…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    86928-plasmid-map-sequence-id-175058

    Enlarge

  12. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91854-plasmid-map-sequence-id-176529

    Enlarge

  13. U6-sgRNA-BbsI-CMV-EGFP cloning vector

    Plasmid
    #239464
    Purpose
    sgRNA cloning vector with EGFP expression cassette. An sgRNA spacer sequence can be cloned into BbsI sites. A CMV-EGFP expression cassette is included as a reporter of transfection efficiency.
    Depositor
    Patrick Harrison
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    239464-plasmid-map-sequence-id-476201

    Enlarge

  14. DsRed2-C1

    Plasmid
    #54611
    Purpose
    Localization: C1 Cloning Vector, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    DsRed2
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54611-plasmid-map-sequence-id-257389

    Enlarge

  15. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118156-plasmid-map-sequence-id-239644

    Enlarge

  17. pC0042-PguCas13b crRNA backbone

    Plasmid
    #103853
    Purpose
    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-PguCas13b DR-BbsI-BbsI-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103853-plasmid-map-sequence-id-199149

    Enlarge

Showing: 1341 - 1360 of 63651 results