162,981 results

1. U6-BbsI-GGsite-mCherry-Puro

   Plasmid

   #221232

   Purpose

   Cloning vector for sgRNA with mCherry and Puro

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. hLRP1-pEZT-BM

   Plasmid

   #177985

   Purpose

   Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

   Depositor

   Lora Hooper

   Article

   Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

   Insert

   LDL receptor related protein 1 (LRP1 Human)
   

   Use

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-AcrIIA4_gb39_lenti

   Plasmid

   #138305

   Purpose

   Lenti expression of AcrIIA4

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastbac1 Nterminal GST

   Plasmid

   #128058

   Purpose

   Baculovirus expression vector with N terminal GST

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-JunWT-Myc

   Plasmid

   #47443

   Purpose

   mammalian expression of WT c-Jun

   Depositor

   Axel Behrens

   Article

   Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

   Insert

   Jun (Jun Mouse)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2859

   Plasmid

   #143386

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ID1 (ID1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBtet-GP

   Plasmid

   #60495

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA3b67Rb

   Plasmid

   #58311

   Purpose

   Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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