Addgene

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pET28a-SET

Plasmid

#234708

Purpose

expresses His-tagged human SET in E coli

Depositor

Lienhard Schmitz

Article

Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

Insert

SET (SET Human)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

lacI

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-NUP98a_IDR-EGFP

Plasmid

#232766

Purpose

Expresses dCas9-NUP98a_IDR-EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

dCas9-NUP98a_IDR-EGFP (NUP98 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSM.wrmScarlet1-10::SL2::GFP1-10

Plasmid

#234318

Purpose

For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

Insert

wrmScarlet1-10::SL2::GFP1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Orai1-jGCaMP8f

Plasmid

#231630

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f (ORAI1 Synthetic, Human)

Use

Tags

jGCaMP8f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mCMVp

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M01-412

Plasmid

#225664

Purpose

Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsCopGFP
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

SORE6-mCMVp and WPRE-SV40p

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0059

Plasmid

#141500

Purpose

Lentiviral vector for overexpressing the CTBP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CTBP1 (CTBP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X FLAG

Plasmid

#225332

Purpose

Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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