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pDONR223 SARS-CoV-2 NSP10

Plasmid

#141264

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 3, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pDONR223 SARS-CoV-2 ORF6

Plasmid

#141275

Purpose

Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLVX-Tight-Puro-KrasG12D2B

Plasmid

#139786

Purpose

Lentiviral tet-inducible expression of KrasG12D (mouse)

Depositor

Joan Massague

Publication

Su et al Nature. 2020 Jan;577(7791):566-571. doi:

Insert

KrasG12D (Kras Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

G12D

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Publication

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Publication

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-PIK3CA-E545K

Plasmid

#116485

Purpose

Lentiviral expression of PIK3CA E545K

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E545K

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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G5-CD40

Plasmid

#121166

Purpose

pMP71 vector expressing GFP - P2A - G5 - myc - CD40

Depositor

Gabriel Victora

Publication

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

G5-CD40

Use

Retroviral

Tags

GFP and myc

Expression

Mammalian

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSGDlucV3.0

Plasmid

#119760

Purpose

dual luciferase with FMDV StopGo sequences flanking MCS

Depositor

John Atkins

Publication

Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV40 and T7

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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