We narrowed to 3,284 results for: cgas

1. T5-sgRNA

   Plasmid

   #113655

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter T5 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-PpU6P-sgRNA-L1L2

   Plasmid

   #113735

   Purpose

   Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROP_C9_Puro_IDH2

   Plasmid

   #183301

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   IDH2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-mtIF3 shRNA_TagRFP657

   Plasmid

   #182367

   Purpose

   shRNA targeting mtIF3 mRNA

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Insert

   mtIF3 (Mtif3 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG3K-U6SC

   Plasmid

   #134754

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA-U6, zCas9-35SC

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK162_mCherry-4.1G-hardened

   Plasmid

   #46361

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   4.1G (EPB41L2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Modified for siRNA resistance to gcTccTcaTttCgaAc…

   Promoter

   CMV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDrop

   Plasmid

   #184872

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA6.2-GW/EmGFP-miR-SUMO23

   Plasmid

   #31073

   Depositor

   Wulf Paschen

   Article

   Yang et al Biochem Biophys Res Commun. 2009 Apr 24.

   Insert

   SUMO2/3 microRNA (SUMO2, SUMO3 Human)
   

   Use

   RNAi

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN-PBAD-sgRNA-A2NT

    Plasmid

    #62257

    Purpose

    expression of A2NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A2NT sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pG3B-U6SC

    Plasmid

    #134753

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-35SC

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOC2 Tubb3-GFP KI

    Plasmid

    #183432

    Purpose

    CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. RSRF-Luc-2mut

    Plasmid

    #31819

    Depositor

    Astar Winoto

    Article

    Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

    Insert

    Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    two CTATATTTAG RSRF sites mutated to CGATATTTCG

    Promoter

    mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

14. PX459-APP-KO

    Plasmid

    #176487

    Purpose

    To knockout APP gene

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TET-pLKO.1 PURO shLuc

    Plasmid

    #83092

    Purpose

    Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pG3B-U3Ub

    Plasmid

    #134750

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA- OsU3, zCas9-Ubi1

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-hU6-mU6-BlasGO

    Plasmid

    #136906

    Purpose

    Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPRv2-sgLmnb2-HepmCherry

    Plasmid

    #192830

    Purpose

    Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBrain-GFP-shGL2

    Plasmid

    #60004

    Purpose

    Co-expresses control GL2 shRNA and GFP.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    EGFP
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U1

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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