We narrowed to 3,574 results for: golden gate

1. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

   Plasmid

   #219711

   Purpose

   Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MUT_S1-N

   Plasmid

   #218398

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MUT_N-H1

   Plasmid

   #218397

   Purpose

   3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK7-g7g8-K8

   Plasmid

   #218220

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK3-g3g4-K4

   Plasmid

   #218218

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK5-g5g6-K6

   Plasmid

   #218219

   Purpose

   Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   AtU6 promoters and sgRNA scaffolds
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4_2WWE

   Plasmid

   #234210

   Purpose

   Heterologous protein expression of PI3K-C2-gamma in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2WWE (PIK3C2G Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219687

   Purpose

   Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPtGG-1

   Plasmid

   #89990

   Purpose

   pBR322-based entry vector for Gateway cloning

   Depositor

   Philip Weyman

   Article

   Bielinski et al PeerJ Preprints. 2017 5:e2907v1

   Type

   Empty backbone

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLII-proUBI10_CKA3 KD K63M,N170D-FLAG_termNOS _OLE-RED

    Plasmid

    #238523

    Purpose

    Plant expression of flag-tagged A. thaliana CK2alpha3 KD K63M,N170D; with OLE1-RED marker

    Depositor

    Michael Hothorn

    Insert

    AtCK2alpha3 (AT2G23080 Mustard Weed)
    

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    K63M N170D

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid

    #239461

    Purpose

    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.

    Depositor

    Alexis Komor

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

    Plasmid

    #228646

    Purpose

    Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    MERS-Cov Mpro domain
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAIP4_6IYE

    Plasmid

    #234237

    Purpose

    Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAIP4_1T3Y

    Plasmid

    #234228

    Purpose

    Heterologous protein expression of coactosin-like protein in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1T3Y (COTL1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAIP4_1JL1

    Plasmid

    #234206

    Purpose

    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1JL1 (rnhA Escherichia coli)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAIP4_3TOW

    Plasmid

    #234201

    Purpose

    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3TOW (MVB12B Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAIP4_1KQX

    Plasmid

    #234181

    Purpose

    Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1KQX (rbp2a Zebrafish)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAIP4_1AKO

    Plasmid

    #234162

    Purpose

    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1AKO (xthA Escherichia coli K-12)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAIP4_4NFB

    Plasmid

    #234161

    Purpose

    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4NFB (PILRA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease

    Plasmid

    #228638

    Purpose

    Bacterial expression of the Coxsackievirus 63 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 63 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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