We narrowed to 24,167 results for: p

1. pSC33

   Plasmid

   #104807

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr8g060730 (Fbl1-like). Also expresses Cas9 from Gmubi promoter.

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr8g060730
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSC25

   Plasmid

   #104799

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101040
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSC24

   Plasmid

   #104798

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101120, Medtr2g101130
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSC22

   Plasmid

   #104796

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g101120, Medtr2g101130
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSC19

   Plasmid

   #104793

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

   Depositor

   Nevin Young

   Article

   Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

   Insert

   Medtr2g020630
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-20b-T7-Ntx

   Plasmid

   #58714

   Purpose

   Expresses T7-Notexin in Escherichia coli

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAB051e

   Plasmid

   #79195

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PExt-10cons lambda op-62 gIII, luxAB; Ptet lambda cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PExt-10cons lambda op-62; Ptet

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAB078d7

   Plasmid

   #79205

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (off-target); Ppro1

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB085e

   Plasmid

   #79212

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ptet

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid

    #206114

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid

    #206082

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV/B-actin

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid

    #206066

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid

    #206096

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV/B-actin

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge