We narrowed to 9,915 results for: pET

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pET28a-Hisx6-Sirt6-S56A

Plasmid

#20278

Depositor

Leonard Guarente

Publication

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Mutation

Serine 56 to Alanine

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pNS20-SpCas9-SNAP

Plasmid

#113717

Purpose

Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

Depositor

Gerald Schwank

Publication

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Publication

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Promoter

T7

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Publication

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP2

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Publication

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-LSSmCherry1(-13)

Plasmid

#219415

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Publication

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

LSSmCherry1(-13)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mBlueberry(-9)

Plasmid

#219416

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Publication

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mBlueberry2(-9)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E, R234E

Promoter

T7

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis -MH)

Plasmid

#195613

Purpose

Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

Depositor

Frederick Gimble

Publication

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET11b-SpyCatcher-M2e

Plasmid

#165990

Purpose

SpyCatcher-fused influenza M2 ectodomain with removable HisTag

Depositor

Vesa Hytönen

Publication

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyCatcher-M2e

Tags

HisTag and SpyCatcher

Expression

Bacterial

Promoter

T7

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21b - S. thermophilus DltB

Plasmid

#132607

Purpose

expresses S. thermophilus DltB in E. coli

Depositor

Wenqing Xu

Publication

Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

Insert

Dltb

Expression

Bacterial

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET32a-His-FADD

Plasmid

#78803

Purpose

To purify His-tagged recombinant FADD protein

Depositor

Jaewhan Song

Insert

GIG3 (FADD Human)

Tags

6x His, S-Tag, Thrombin cleavage site, and TrxA (…

Expression

Bacterial

Promoter

T7

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
K12/A322+His_pET-28a(+)

Plasmid

#255042

Purpose

Recombinant tau construct for seeding activity assays and other applications.

Depositor

Byron Caughey

Publication

Santambrogio et al Alzheimers Dement. 2026 Feb;22(2):e71112

Insert

modified tau protein (K12) (MAPT Human)

Expression

Bacterial

Mutation

The K12 tau fragment (residues 244-275 and 306-40…

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pET-30a-METTL17-8XHIS

Plasmid

#215705

Purpose

Express METTL17-8XHIS in C41(DE3) cells

Depositor

Vamsi Mootha

Publication

Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

Insert

METTL17 (METTL17 Human)

Tags

HIS

Expression

Bacterial

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-HLA-A0201-ST3

Plasmid

#223220

Purpose

Expression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteria

Depositor

Omer Dushek

Publication

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

HLA--A2 heavy chain (HLA-A Human)

Tags

Spytag

Expression

Bacterial

Promoter

T7

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Publication

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and p53(1-85)

Expression

Bacterial

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-17b_Unc104-KIF5B-HALO

Plasmid

#169996

Purpose

Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

Depositor

Ron Vale

Publication

Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

Insert

Kinesin-like protein Unc-104 fused with KIF5B

Tags

HALO-tag, 6xHIS

Expression

Bacterial

Promoter

T7

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)-DL4

Plasmid

#199168

Purpose

used to express a supercharged GFP variant that forms inclusion bodies in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)-DL4

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag141/145

Plasmid

#194660

Purpose

Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag141/145

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHaloTag154/156

Plasmid

#194661

Purpose

Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHaloTag154/156

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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