We narrowed to 6,923 results for: pcdna3.1

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pY008 (pcDNA3.1-hPbCpf1)

Plasmid

#69980

Purpose

Expresses humanized LPbCpf1

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1 Flag-Sesn2

Plasmid

#61868

Purpose

Transient overexpression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc-PITPalpha

Plasmid

#58277

Purpose

mammalian expression of human PITP alpha

Depositor

Shamshad Cockcroft

Insert

PITP alpha (PITPNA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.1 AT1AR-324delta

Plasmid

#45635

Depositor

Robert Lefkowitz

Publication

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-324delta (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

added stop codon at 324 aa

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Publication

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-GFP-EphrinB3-Flag

Plasmid

#155012

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

Depositor

Sinisa Urban

Publication

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

Plasmid

#183252

Purpose

Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

anti-CD19 scFvFc (FMC-63)

Expression

Mammalian

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-2A-eGFP(W58X) pcDNA3.1(-)

Plasmid

#172229

Purpose

Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

Depositor

Joshua Rosenthal

Publication

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

mCherry-2A-eGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-Cre

Plasmid

#167939

Purpose

human CD81-TEV cleavage site-Cre

Depositor

Masaharu Somiya

Publication

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

Cre recombinase and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Publication

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ost4-GFP-MTn

Plasmid

#154351

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Publication

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

GFP

Expression

Mammalian

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-BirA*-KRASG12V

Plasmid

#122208

Purpose

Expresses myc-BirA* fused to KRASG12V in mammalian cells

Depositor

Christopher Counter

Publication

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

KRASG12V (KRAS Human)

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tat101(AD8)-Flag

Plasmid

#115777

Purpose

pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

Depositor

Damian Purcell

Publication

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Tat protein (AD8)

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-KLF12/pcDNA3.1-Neo

Plasmid

#81069

Purpose

Expression of epitope-tagged human KLF12 in mammalian cells.

Depositor

Julian Downward

Publication

Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

Insert

FLAG-KLF12 (KLF12 Human)

Tags

FLAG

Expression

Mammalian

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-delPAZ

Plasmid

#51367

Depositor

Narry Kim

Publication

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to PAZ domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(133) pcDNA3.1-Nup62-Flag-EPEA

Plasmid

#160744

Purpose

Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

Depositor

Christina Woo

Publication

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

Nup62-Flag-EPEA

Tags

EPEA and Flag

Expression

Mammalian

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

Plasmid

#18017

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V ECFP-W67G

Tags

ECFP-W67G and Venus

Expression

Mammalian

Mutation

mglB-D154A; non-functional ECFP

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
CRAF_S29A_pcDNA3.1

Plasmid

#86506

Purpose

Expresses CRAF_S29A in mammalian cells [Edit]

Depositor

Dustin Maly

Publication

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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