165,567 results

1. pCDB327

   Plasmid

   #113671

   Purpose

   expresses Ulp1_R3 in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Ulp1_R3
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   contains solubility enhancing mutations

   Promoter

   T7

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. T-2A-EGFP-PGK-Puro

   Plasmid

   #83344

   Purpose

   Donor vector to knocin EGFP into human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   Human Brachyury knockin homology arms (TBXT Human)
   

   Tags

   2A-EGFP

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDA_526

   Plasmid

   #184859

   Purpose

   Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

   Plasmid

   #160141

   Purpose

   Cre- dependent expression of Sun1GFP for nuclei isolation

   Depositor

   Andreas Pfenning

   Article

   Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

   Insert

   Sun1-GFP (Sun1 Mouse)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N-truncated: removed amino acids 1-207, retained …

   Promoter

   Ef1a

   Available Since

   March 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-3'3xFlag

   Plasmid

   #208616

   Purpose

   Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-BAZ1B

   Plasmid

   #65372

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1B (BAZ1B Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-KP1

   Plasmid

   #206504

   Purpose

   AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   KP1 capsid
   

   Use

   AAV

   Promoter

   p5

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50464

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge