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PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

Plasmid

#172226

Purpose

Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

SOX17 (SOX17 Human)

Use

Piggybac transposon

Tags

3XFLAG, DD-domain, GS-linker, and TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-NDUFB2

Plasmid

#139841

Purpose

Lentiviral expression of human NDUFB2

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

NDUFB2 (NDUFB2 Human)

Use

Lentiviral

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_20-7

Plasmid

#201974

Purpose

Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_20-7

Expression

Mammalian

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-tmpknot-epegRNA

Plasmid

#214091

Purpose

Lentiviral vector expressing epegRNA to induce GBA N370S mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

GBA N370S epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

Plasmid

#191397

Purpose

sgRNA targeting alpha-satellites on human chromosome 9

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

sgRNA targeting alpha satellites on human chromosome 9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP301-pAAV-CMV-MCS3-pA

Plasmid

#113676

Purpose

CMV driven Multi Cloning Site-3

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

Plasmid

#210487

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pKB12 (pDEST-DHFR F[3] C-term, HygR)

Plasmid

#210486

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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