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pBMN YFP-FKBP(DD)

Plasmid

#31766

Depositor

Thomas Wandless

Article

Chu et al Bioorg Med Chem Lett. 2008 Nov 15;18(22)

Insert

HA-YFP-FKBP(DD) (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

E31G, F36V, R71G and K105E on FKBP12

Promoter

LTR

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pSH1/M-Fv'-Fvls-E

Plasmid

#15273

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36) (FKBP1A Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12 F36-->V 1st FKBP uses "wobbled&qu…

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pBMN FKBP(DD)-YFP

Plasmid

#31763

Depositor

Thomas Wandless

Article

Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

Insert

FKBP(DD)-YFP-HA (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

F36V, L106P on FKBP12

Promoter

LTR

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-TOP luciferase

Plasmid

#26611

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

eEF2 5'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pJD427

Plasmid

#154371

Purpose

Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

Depositor

Joseph Davis

Article

Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

Insert

proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-FKBP (CF)

Plasmid

#20160

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

CFP-FKBP (FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pUC19-eGFP-P2A-dTAG-Ing3

Plasmid

#170754

Purpose

Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

eGFP-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

eGFP-P2A-2xHA-dTAG

Expression

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

none

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Expression

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)

Use

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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