3,636 results

1. K9L mutant of H3K9me3 biosensor

   Plasmid

   #120807

   Purpose

   FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
   

   Expression

   Mammalian

   Mutation

   lysine 9 is mutated to leucine 9 on histone H3

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUAST-yki:FLAG(3x)

   Plasmid

   #85622

   Purpose

   Express 3x Flagged yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Development. 2008 Mar;135(6):1081-8. doi

   Insert

   yki (yki Fly)
   

   Tags

   3x FLAG

   Expression

   Insect

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD5-U6-3-t-attP40

   Plasmid

   #133561

   Purpose

   gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   attP40 region guide RNAs
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASp YFP Ras85D CA

   Plasmid

   #52247

   Purpose

   Drosophila expression of YFP-Ras85D constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

   Plasmid

   #217512

   Purpose

   KD Recombinase dependent conditional smGdP-10XOllas cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
   

   Tags

   smGdP-10XOllas

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Expression

   Insect

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc-mCh-Tub

   Plasmid

   #24288

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Alpha-Tubulin (alphaTub84B Fly)
   

   Tags

   mCherry

   Expression

   Insect

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pScarlessHD-DsRed-w+

   Plasmid

   #80801

   Purpose

   Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.

   Depositor

   Kate O'Connor-Giles

   Inserts

   piggyBac-3xP3-DsRed
   GMR-white
   

   Use

   High copy, amp resistance

   Promoter

   3xP3 and GMR::hsp70

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

   Plasmid

   #63169

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-FLAG"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Expression

    Insect

    Mutation

    S129A

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABR IRFP-Bcd-LEXY

    Plasmid

    #182595

    Purpose

    Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Tags

    IRFP and LEXY

    Expression

    Insect

    Promoter

    maternal tubulin

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NCD-GFP

    Plasmid

    #24507

    Depositor

    Ron Vale

    Article

    Case et al Cell. 1997 Sep 5. 90(5):959-66.

    Insert

    NCD (ncd Fly)
    

    Tags

    GFP and His

    Expression

    Bacterial

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pOpen-dromedNK

    Plasmid

    #165579

    Purpose

    Used for dNTP synthesis

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    dNK from drosophila
    

    Use

    Synthetic Biology

    Available Since

    Aug. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAc5.1A-Dm-miR-92a

    Plasmid

    #22767

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

    Insert

    micro RNA 92a (mir-92a primary transcript, Fly)
    

    Use

    Drosophila expression

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. 10XSTAT92E–luciferase

    Plasmid

    #37393

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    SOCS36E enhancer fragment (Socs36E Fly)
    

    Use

    Luciferase; Jak/stat reporter construct

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB1H1

    Plasmid

    #12611

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Dorsal RHR in pB1H1 (dl Fly)
    

    Tags

    flag

    Expression

    Bacterial

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-AlstR-IRES2-EGFP

    Plasmid

    #14895

    Depositor

    Edward Callaway

    Article

    Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

    Insert

    AlstR (AstA-R1 Fly)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHD-DsRed-w+

    Plasmid

    #80926

    Purpose

    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.

    Depositor

    Kate O'Connor-Giles

    Inserts

    LoxP-3xP3-DsRed-LoxP
    GMR-white
    

    Use

    High copy, amp resistance

    Promoter

    3xP3 and GMR::hsp70

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159676

    Purpose

    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ909

    Plasmid

    #44480

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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