Purpose
              
              VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
             
          
          
          
            
          
        
          
  
  
    
      
        Inserts
      
      
        
          tnsA
        
          tnsB
        
          tnsC
        
          tnsD
        
          tniQ
        
          cas8-cas5 fusion
        
          cas7
        
          cas6
        
          crRNA
        
          Tn7 transposon
        
          2xLguI Cargo Stuffer
        
          SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
        
      
    
   
  
  
  
  
    
  
  
  
    
  
         
          
            
              
                Available Since
              
              
                Sept. 9, 2022
              
             
          
          
            
              Availability
            
            
              
                Academic Institutions and Nonprofits only