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We narrowed to 619 results for: ubiquitin plasmid

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Showing: 301 - 350 of 619 results

  1. pUltra-Exon1Q23-Myc-B

    Plasmid
    #110488
    Purpose
    Lentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    short 3'UTR mutant huntingtin exon1 fragment…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110488-plasmid-map-sequence-id-212609

    Enlarge

  8. pUltra-hot-H2B-mTagBFP2

    Plasmid
    #161792
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    mCherry and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161792-plasmid-map-sequence-id-316815

    Enlarge

  9. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158162-plasmid-map-sequence-id-314581

    Enlarge

  10. p667-UBC-CDK12-V5-TurboID_IDG-K

    Plasmid
    #135244
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-TurboID (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135244-plasmid-map-sequence-id-311575

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  11. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid
    #158160
    Purpose
    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-3xHA-miniTurbo-3XNLS
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158160-plasmid-map-sequence-id-309971

    Enlarge

  12. p667-UBC-CDK12-V5_IDG-K

    Plasmid
    #135245
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135245-plasmid-map-sequence-id-268084

    Enlarge

  13. p663-UBC-miniTurbo-V5-CDK13_IDG-K

    Plasmid
    #135266
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135266-plasmid-map-sequence-id-268080

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  14. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid
    #131134
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-MCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131134-plasmid-map-sequence-id-255499

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  18. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

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  19. p667-UBC-NEK7-V5-miniTurbo_IDG-K

    Plasmid
    #189882
    Purpose
    Gateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK7 (NEK7 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    189882-plasmid-map-sequence-id-374826

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  20. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid
    #189871
    Purpose
    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK1 (NEK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189871-plasmid-map-sequence-id-374462

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  21. p663-UBC-miniTurbo-V5-NEK6_IDG-K

    Plasmid
    #189872
    Purpose
    Gateway destination clone of NEK6 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK6 (NEK6 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189872-plasmid-map-sequence-id-374315

    Enlarge

  22. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid
    #135243
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-miniTurbo (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135243-plasmid-map-sequence-id-269114

    Enlarge

  23. p667-UBC-GFP-V5_IDG-K

    Plasmid
    #135242
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135242-plasmid-map-sequence-id-270199

    Enlarge

  24. pUltra-Exon1Q145-Myc-B

    Plasmid
    #110489
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110489-plasmid-map-sequence-id-287962

    Enlarge

  25. pAAV-UbC-mEmerald Rab7a

    Plasmid
    #115238
    Purpose
    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab7a fused to mEmerald (RAB7A Human)
    Use
    AAV
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    7 Amino Acids (21 Nucleotides) between mEmerald a…
    Promoter
    Human UbC
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    115238-plasmid-map-sequence-id-222080

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  28. p667-UBC-SCYL1-V5-miniTurbo_IDG-K

    Plasmid
    #189911
    Purpose
    Gateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    SCYL1 (SCYL1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189911-plasmid-map-sequence-id-374157

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  29. p663-UBC-miniTurbo-V5-MARK1_IDG-K

    Plasmid
    #170582
    Purpose
    Gateway destination clone of MARK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-MARK1 (MARK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170582-plasmid-map-sequence-id-341639

    Enlarge

  30. p663-UBC-miniTurbo-V5-CDK15_IDG-K

    Plasmid
    #158179
    Purpose
    Gateway destination clone of CDK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK15 (CDK15 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    158179-plasmid-map-sequence-id-310247

    Enlarge

  31. p667-UBC-PKMYT1-V5-miniTurbo_IDG-K

    Plasmid
    #135246
    Purpose
    Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PKMYT1-V5-miniTurbo (PKMYT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135246-plasmid-map-sequence-id-311576

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  32. p663-UBC-V5-GSK3B_IDG-K

    Plasmid
    #158166
    Purpose
    Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-GSK3B (GSK3B Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158166-plasmid-map-sequence-id-309973

    Enlarge

  33. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid
    #135272
    Purpose
    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-BRSK2 (BRSK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135272-plasmid-map-sequence-id-269112

    Enlarge

  34. p663-UBC-V5-BRSK1_IDG-K

    Plasmid
    #135270
    Purpose
    Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-BRSK1 (BRSK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135270-plasmid-map-sequence-id-268548

    Enlarge

  35. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

    Enlarge

  36. pAAV-UbC-mCherry Rab5

    Plasmid
    #115239
    Purpose
    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.
    Depositor
    Pantelis Tsoulfas
    Insert
    Rab5a (RAB5A Homo sapiens, Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human UbC
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115239-plasmid-map-sequence-id-222083

    Enlarge

  41. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

    Enlarge

  44. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

    Enlarge

  45. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198046
    Purpose
    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198046-plasmid-map-sequence-id-383687

    Enlarge

  46. p667-UBC-CDC42BPB-V5-miniTurbo_IDG-K

    Plasmid
    #198008
    Purpose
    Gateway destination clone of CDC42BPB (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDC42BPB-V5-miniTurbo (CDC42BPB Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    198008-plasmid-map-sequence-id-390038

    Enlarge

  47. p663-UBC-miniTurbo-V5-NEK10_IDG-K

    Plasmid
    #189874
    Purpose
    Gateway destination clone of NEK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK10 (NEK10 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    189874-plasmid-map-sequence-id-381797

    Enlarge

  48. p663-UBC-miniTurbo-V5-CDK20_IDG-K

    Plasmid
    #189883
    Purpose
    Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK20 (CDK20 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189883-plasmid-map-sequence-id-374321

    Enlarge

  49. p663-UBC-miniTurbo-V5-TTBK2_IDG-K

    Plasmid
    #189881
    Purpose
    Gateway destination clone of TTBK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TTBK2 (TTBK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189881-plasmid-map-sequence-id-374318

    Enlarge

  50. p663-UBC-miniTurbo-V5-NEK4_IDG-K

    Plasmid
    #189875
    Purpose
    Gateway destination clone of NEK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK4 (NEK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189875-plasmid-map-sequence-id-374316

    Enlarge

Showing: 301 - 350 of 619 results