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Plasmid#123319PurposeLentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 G40N-Puro
Plasmid#123320PurposeLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 40 to AsparaginePromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 G40N-P2A-Blast
Plasmid#123321PurposeLentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 40 to AsparaginePromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CSNK1A1L_IDG-K
Plasmid#170591PurposeGateway destination clone of CSNK1A1L (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CSNK1A1L (CSNK1A1L Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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CSNK1A1L gRNA (BRDN0001145992)
Plasmid#77381Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1A1LDepositorInsertCSNK1A1L (CSNK1A1L Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1L gRNA (BRDN0001146686)
Plasmid#77382Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1A1LDepositorInsertCSNK1A1L (CSNK1A1L Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1L gRNA (BRDN0001147607)
Plasmid#77383Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1A1LDepositorInsertCSNK1A1L (CSNK1A1L Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FuPick-shRNA
Plasmid#31614DepositorInsertPICK1 shRNA (Pick1 Rat)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_V5-CSNK1A1
Plasmid#92014PurposeExpresses V5-CSNK1A1, includes IRES-mCherryDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1 gRNA (BRDN0001145680)
Plasmid#76189Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1A1DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1 gRNA (BRDN0001145896)
Plasmid#76190Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1A1DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSNK1A1 gRNA (BRDN0001149315)
Plasmid#76188Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK1A1DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nck-SH2
Plasmid#46457DepositorInsertNck (NCK1 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 275-377PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIL-1L
Plasmid#50016PurposeMammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertRanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 full-length (1–510)
Plasmid#193858PurposeExpression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationCodon optimised for expression in E. coliPromoterT7Available sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 C3_25
Plasmid#60305PurposeThis plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.DepositorInsertROCK1 enhancer (ROCK1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR D-TOPO-C3_25
Plasmid#60268PurposeThis plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.DepositorInsertROCK1 enhancer (ROCK1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 helix-RBR (233–510)
Plasmid#193859PurposeExpression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationResidues 233–510, codon optimised for expression …PromoterT7Available sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only