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K20‐scFv‐pSMBP2

Plasmid

#159940

Purpose

To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

Depositor

Sandra Schmid

Article

Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

Insert

anti beta-1 integrin scFv

Use

Tags

6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Timothy Springer, Pontus Nordenfelt

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 siRNA

Plasmid

#16266

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SIRNA Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Hygro(-)-B2-cT

Plasmid

#104355

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Timothy Springer, Pontus Nordenfelt

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Timothy Springer, Pontus Nordenfelt

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 SCR

Plasmid

#16267

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SCR Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pENTR221-Illusia(YYEE)

Plasmid

#222591

Purpose

Gateway entry vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYEE)

Use

Gateway entry vector

Tags

Expression

Mutation

Mutations in the NPxY sites equivolent to Y783E, …

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia(YYEE)

Plasmid

#222594

Purpose

Piggybac expression vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYEE)

Use

Transposon-based stable expression

Tags

Expression

Mammalian

Mutation

Mutations in the NPxY sites equivolent to Y783E, …

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(Y783F)

Plasmid

#222595

Purpose

Gateway entry vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y783F)

Use

Gateway entry vector

Tags

Expression

Mutation

Mutation in the NPxY(783) site equivolent to Y783…

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia(Y783F)

Plasmid

#222596

Purpose

Piggybac expression vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y783F)

Use

Transposon-based stable expression

Tags

Expression

Mammalian

Mutation

Mutation in the NPxY(783) site equivolent to Y783…

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(Y795F)

Plasmid

#222597

Purpose

Gateway entry vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y795F)

Use

Gateway entry vector

Tags

Expression

Mutation

Mutation in the NPxY(795) site equivolent to Y795…

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia(Y795F)

Plasmid

#222598

Purpose

Piggybac expression vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(Y795F)

Use

Transposon-based stable expression

Tags

Expression

Mammalian

Mutation

Mutation in the NPxY(795) site equivolent to Y795…

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Illusia(YYFF)

Plasmid

#215443

Purpose

Gateway entry vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYFF)

Use

Gateway entry vector

Tags

Expression

Mutation

Mutations in the NPxY sites equivolent to Y783F, …

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Beta1-GFP in pHcgreen

Plasmid

#69804

Purpose

Expresses Beta1-GFP integrin for normal cell transfection

Depositor

Martin Humphries

Article

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1A integrin (ITGB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Beta1-GFP pFB-Neo

Plasmid

#69767

Purpose

Expresses Beta1-GFP integrin for retroviral transfection

Depositor

Martin Humphries

Article

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1 integrin (ITGB1 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

IRES

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
mouse b3 full length

Plasmid

#217814

Purpose

Full length mouse integrin b3 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB3 (Itgb3 Mouse)

Use

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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mouse av full length

Plasmid

#217809

Purpose

Full length mouse integrin av expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGAV (Itgav Mouse)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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mouse a2b full length

Plasmid

#217812

Purpose

Full length mouse integrin a2b expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA2B (Itga2b Mouse)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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mouse a5 full length

Plasmid

#217810

Purpose

Full length mouse integrin a5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA5 (Itga5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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human a5 full length

Plasmid

#217818

Purpose

Full length human integrin a5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA5 (ITGA5 Human)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge