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pcDNA3.2 mir-1-1 reporter dme-mir-125

Plasmid

#46667

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-125

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir-205

Plasmid

#46679

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-205 (MIR205 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter hsa-mir-142

Plasmid

#46678

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-142 (MIR142 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-mir-240

Plasmid

#46661

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-240

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-mir-235

Plasmid

#46660

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-235

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-mir-124

Plasmid

#46659

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-124

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter dme-mir-279

Plasmid

#46668

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-279

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter dme-mir-92a

Plasmid

#46666

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-92a

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Roger Tsien, Erik Rodriguez

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-smURFP-IRES-HO-1

Plasmid

#80345

Purpose

Expresses smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Roger Tsien, Erik Rodriguez

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Smac(1-60)mCherry

Plasmid

#40880

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac(1-60)mCherry (DIABLO Human)

Use

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 c-SRC (1-249)

Plasmid

#42204

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-249) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-249

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
K44A HA-dynamin 1 pcDNA3.1

Plasmid

#34683

Depositor

Sandra Schmid

Insert

dynamin 1ba (DNM1L Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

K44A

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-miR-16-1

Plasmid

#51382

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

MIR16-1 microRNA 16-1 (MIR16-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 c-SRC (1-397)

Plasmid

#42205

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-397) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-397

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-let-7a-1

Plasmid

#51377

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7a-1 (MIRLET7A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SARS-1-RBD-8h

Plasmid

#161821

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Receptor-binding domain (RBD) of spike protein S

Use

Tags

GSG linker and 8his tag and HA leader sequence

Expression

Mammalian

Mutation

Human codon optimized RBD (a.a. T320-D518)

Promoter

CMV

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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