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We narrowed to 527 results for: plasmid cloning by pcr

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Showing: 121 - 140 of 527 results

  5. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80811-plasmid-map-sequence-id-152800

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  6. pScarlessHD-3xFLAG-DsRed

    Plasmid
    #80820
    Purpose
    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    3xFLAG-piggyBac-DsRed
    Use
    Unspecified
    Tags
    3xFLAG
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80820-plasmid-map-sequence-id-152798

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  7. pScarlessHD-2xHA-DsRed

    Plasmid
    #80822
    Purpose
    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    2xHA-piggyBac-DsRed
    Use
    Unspecified
    Tags
    2xHA
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80822-plasmid-map-sequence-id-152787

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  9. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Kevin White
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Mutation
    rs4864608
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113807-plasmid-map-sequence-id-231037

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  10. pNR1D2-donor

    Plasmid
    #112314
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP
    Depositor
    Kevin White
    Insert
    NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
    Use
    CRISPR
    Mutation
    hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112314-plasmid-map-sequence-id-228473

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  11. pCCL-c-MNDU3-CD3D-274+5G>A

    Plasmid
    #198023
    Purpose
    LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
    Depositor
    Donald Kohn
    Insert
    CD3D c.274+5G>A
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198023-plasmid-map-sequence-id-390930

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  12. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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  13. pECM1

    Plasmid
    #75445
    Purpose
    Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75445-plasmid-map-sequence-id-146473

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  14. pECT1

    Plasmid
    #75446
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75446-plasmid-map-sequence-id-146474

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  15. pECT1B

    Plasmid
    #75447
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1B
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75447-plasmid-map-sequence-id-146475

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  16. pECT1D

    Plasmid
    #75448
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1D
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75448-plasmid-map-sequence-id-146476

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  17. pECT1E

    Plasmid
    #75449
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1E
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75449-plasmid-map-sequence-id-146477

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  18. pATF4-donor

    Plasmid
    #113805
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP
    Depositor
    Kevin White
    Insert
    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    Use
    CRISPR
    Mutation
    rs140428747
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113805-plasmid-map-sequence-id-231035

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  19. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

    Plasmid
    #198022
    Purpose
    LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population
    Depositor
    Donald Kohn
    Insert
    CD3D c.202C>T-cDNA
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198022-plasmid-map-sequence-id-390932

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  20. pCCL-c-MNDU3-CD3D-275-2A>G

    Plasmid
    #198024
    Purpose
    LV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in Japan
    Depositor
    Donald Kohn
    Insert
    CD3D c.275-2A>G
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198024-plasmid-map-sequence-id-390335

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Showing: 121 - 140 of 527 results