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Plasmid#137088PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFPDepositorInserttagRFP
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-sfGFP-DsRed
Plasmid#80811PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertsfGFP-piggyBac-DsRed
UseUnspecifiedTagssfGFPExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-3xFLAG-DsRed
Plasmid#80820PurposeVector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xFLAG-piggyBac-DsRed
UseUnspecifiedTags3xFLAGExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-2xHA-DsRed
Plasmid#80822PurposeVector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert2xHA-piggyBac-DsRed
UseUnspecifiedTags2xHAExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREST-donor
Plasmid#113807PurposeCRISPR donor plasmid to tag human transcription factor REST with GFPDepositorInsertREST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
UseCRISPRTagsExpressionMutationrs4864608PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR1D2-donor
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRTagsExpressionMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-274+5G>A
Plasmid#198023PurposeLV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in EcuadorDepositorInsertCD3D c.274+5G>A
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECT3
Plasmid#75450PurposePlasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt3
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECM1
Plasmid#75445PurposePlasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1
Plasmid#75446PurposePlasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1B
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRTagsExpressionMutationrs140428747PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D c.202C>T-cDNA
Plasmid#198022PurposeLV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite populationDepositorInsertCD3D c.202C>T-cDNA
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-275-2A>G
Plasmid#198024PurposeLV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in JapanDepositorInsertCD3D c.275-2A>G
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGJJ055
Plasmid#183622PurposeddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRTagsExpressionMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUNB-donor
Plasmid#112313PurposeCRISPR donor plasmid to tag human transcription factor JUNB with GFPDepositorInsertJUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
UseCRISPRTagsExpressionMutationhg38:19:12792992:C>G, hg38:19:12793018:T>G,…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)
Plasmid#44083DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only