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Smarca4-EGFP knockin donor template

Plasmid

#259654

Purpose

Homologous recombination donor template to generate Smarca4-EGFP knockin mouse embryonic stem cells

Depositor

Charles Roberts

Article

Zhu et al Nature. 2023 Jun;618(7963):180-187. doi:

Insert

Smarca4 (SMARCA4 Human)

Use

Mouse Targeting

Available Since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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Smarce1-EGFP knockin donor template

Plasmid

#259649

Purpose

Homologous recombination donor template to generate Smarce1-EGFP knockin mouse embryonic stem cells

Depositor

Charles Roberts

Article

Zhu et al Nature. 2023 Jun;618(7963):180-187. doi:

Insert

Smarce1 (Smarce1 Mouse)

Use

Mouse Targeting

Available Since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pHnS KI;Actb Donor;3xV5 KO;Template

Plasmid

#240291

Purpose

KI:Actb Donor:3xV5 KO:Template

Depositor

Yuki Ogawa

Insert

KI gRNA for Actb

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHnS KI;Sptbn4 Donor;3xV5 KO;Template

Plasmid

#240295

Purpose

KI:Sptbn4 Donor:3xV5 KO:Template

Depositor

Yuki Ogawa

Insert

KI gRNA for Sptbn4

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHnS KI;Gria1 Donor;3xV5 KO;Template

Plasmid

#240293

Purpose

KI:Gria1 Donor:3xV5 KO:Template

Depositor

Yuki Ogawa

Insert

KI gRNA for Gria1

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHnS KI;Pdha1 Donor;3xV5 KO;Template

Plasmid

#240300

Purpose

KI:Pdha1 Donor:3xV5 KO:Template

Depositor

Yuki Ogawa

Insert

KI gRNA for Pdha1

Use

AAV

Mutation

NA

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHnS KI;Lmnb1 Donor;3xV5 KO;Template

Plasmid

#240297

Purpose

KI:Lmnb1 Donor:3xV5 KO:Template

Depositor

Yuki Ogawa

Insert

KI gRNA for Lmnnb1

Use

AAV

Mutation

NA

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for HCT116

Plasmid

#238245

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-platypus_min2

Plasmid

#173967

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPD027 Reporter Template v1 (Near Spaced)

Plasmid

#138751

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

Plasmid

#167971

Purpose

Tp63 reporter targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

Tp63 (TP63 Human)

Use

CRISPR; Donor template

Tags

Venus

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

Plasmid

#214998

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

Plasmid

#214999

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

Plasmid

#167943

Purpose

SFTPC reporter targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SFTPC (SFTPC Human)

Use

CRISPR; Donor template

Tags

Venus

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

EF1a

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Smarce1-mini-AID knockin donor template

Plasmid

#259652

Purpose

Homologous recombination donor template to generate Smarce1-mini-AID knockin mouse embryonic stem cells

Depositor

Charles Roberts

Article

Zhu et al Nature. 2023 Jun;618(7963):180-187. doi:

Insert

Smarce1 (Smarce1 Mouse)

Use

Mouse Targeting

Available Since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pHnS 2comp Donor;smFP-V5 KO;Template ORF2

Plasmid

#240306

Purpose

Donor:smFP-V5 KO:Template

Depositor

Yuki Ogawa

Insert

Template gRNA

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHnS 2comp Donor;smFP-V5 KO;Template ORF1

Plasmid

#240305

Purpose

Donor:smFP-V5 KO:Template

Depositor

Yuki Ogawa

Insert

Template gRNA

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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