We narrowed to 5,868 results for: nls

1. paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

   Plasmid

   #34911

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   presynaptic mGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Col1A2:TFPnls-T2a-mErt-Cre-Ert

   Plasmid

   #111151

   Purpose

   Fibroblasts specific inducible Cre

   Depositor

   Elly Tanaka

   Insert

   TFPnls-T2a-mErt-Cre-Ert
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   wt

   Promoter

   Col1A2

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

   Plasmid

   #132543

   Purpose

   Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   cmv d0

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. tetO-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69545

   Purpose

   Tet-On system expressing GFP tagged with 2x nuclear localization signals.

   Depositor

   Charles Gersbach

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51085

   Purpose

   Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde and AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin1

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEG302 22aa SunTag NtDRMcd (noNLS) nog

   Plasmid

   #119554

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

   Plasmid

   #51086

   Purpose

   Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa 1.3 kb

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

   Plasmid

   #113899

   Purpose

   Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   TetR-HA-NLS-P2A-BSD-Myc
   

   Use

   Lentiviral

   Tags

   HA-Myc

   Expression

   Mutation

   Promoter

   CMV-MIE

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

    Plasmid

    #136262

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51084

    Purpose

    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2 8xSTAR-sTomato-NLS. PGK-puro

    Plasmid

    #136261

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-eGFP-P2A-LgBiT-NLS

    Plasmid

    #187834

    Purpose

    Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    eGFP-P2A-LgBiT-NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-MCS-MED1long-IDR-YFP-NLS

    Plasmid

    #145276

    Purpose

    Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MED1-IDR long (MED1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid

    #89993

    Purpose

    Donor template for generation of SOX9-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

    Plasmid

    #110057

    Purpose

    Per2 transcription fluorescence reporter

    Depositor

    Eric Erquan Zhang

    Article

    Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    Per2 promoter and Venus (Per2 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    NLS-D2

    Expression

    Mammalian

    Mutation

    Promoter

    Per2

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire

    Plasmid

    #203658

    Purpose

    Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

    Plasmid

    #84604

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

    Plasmid

    #145027

    Purpose

    Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-Tq-Ca-FLITS
    

    Use

    Lentiviral

    Tags

    3xnls-Tq-Ca-FLITS and H2B-Maroon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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