We narrowed to 10,107 results for: ada

1. pMJ114

   Plasmid

   #85995

   Purpose

   One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   modified bovine U6-2

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH0001

   Plasmid

   #85969

   Purpose

   UCOE-SFFV-dCas9-BFP-KRAB

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BFP-KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   SFFV

   Available Since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-eNme2-T.1-ABE8e

   Plasmid

   #185668

   Purpose

   Expresses eNme2-T.1-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOD2044-intDEG

   Plasmid

   #89366

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Promoter

   Pelt-2

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-HA-FbxO5-pcDNA3.1-

   Plasmid

   #52509

   Purpose

   expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO5 (FBXO5 Human, FbxO5)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-eNme2-T.2-ABE8e

   Plasmid

   #185669

   Purpose

   Expresses eNme2-T.2-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-EIN-AlkBH5(66-292)

   Plasmid

   #125732

   Purpose

   Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   AlkBH5(66-292) (ALKBH5 Human)
   

   Tags

   6xHis and EIN

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOD1988-epiDEG

   Plasmid

   #89357

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Promoter

   Pdpy-7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-BLM DM

   Plasmid

   #80071

   Purpose

   Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

   Depositor

   Nathan Ellis

   Article

   Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

   Insert

   BLM (BLM Human)
   

   Tags

   GFP-BLM

   Expression

   Mammalian

   Mutation

   K317R (AAA>AGA) and K331R (AAA>AGA)

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRY2clust-mCherry-RANK

    Plasmid

    #208612

    Purpose

    Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)

    Depositor

    Tomohiro Ishii, Takao Nakata

    Article

    Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

    Insert

    CRY2clust-mCherry-RANK (Cry2 Mouse, Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5-TEX264-deltaLRR-cSSH

    Plasmid

    #220210

    Purpose

    Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    c.4-75del / p.2-24del

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdCMV/V5-DEST-STAT3C-3xFlag

    Plasmid

    #99264

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    A662C N664C

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GDNF-SHC

    Plasmid

    #139624

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GDNF (GDNF Human)
    

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-somAion-citrine

    Plasmid

    #192570

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    somAion
    

    Use

    AAV

    Tags

    Citrine - soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FXYD2-luc

    Plasmid

    #31056

    Depositor

    Gerhart Ryffel

    Article

    Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

    Insert

    FXYD2 (FXYD2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    FXYD2 promoter from -2382 to -1

    Available Since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAdCMV/V5-DEST-S727A-STAT3-3xFlag

    Plasmid

    #99262

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    S727A

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAdCMV/V5-DEST-S727D-STAT3-3xFlag

    Plasmid

    #99263

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3 (S727D)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - S727D (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    S727D

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOD2087-trnDEG

    Plasmid

    #89369

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Promoter

    Pmec-18

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmKikGR Actin

    Plasmid

    #105315

    Purpose

    Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

    Depositor

    Kenneth Yamada

    Article

    Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

    Insert

    actin beta (ACTB Human)
    

    Tags

    KikGR

    Expression

    Mammalian

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. [UBC][EIF4e][GG cloning]

    Plasmid

    #97189

    Purpose

    EIF4e plasmid with Pum cloning site

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    EIF4E variant 1 (EIF4E Human)
    

    Use

    Synthetic Biology

    Tags

    empty Pum cloning site

    Expression

    Mammalian

    Promoter

    UBC (ubiquitin C)

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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