We narrowed to 16,109 results for: form

1. pT3-EF1aH-PIK3CA H1047R

   Plasmid

   #180031

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.

   Depositor

   Xin Chen

   Article

   Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   H1047R

   Promoter

   EF1a

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPC1-hVAP-A KD/MD

   Plasmid

   #104449

   Purpose

   Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K94 to D and M96 to D

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1-hVAP-B KD/MD

   Plasmid

   #104450

   Purpose

   Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   changed K87 to D and M89 to D

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

   Plasmid

   #136684

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLgw Cbx1-V5-EcoDam

   Plasmid

   #59211

   Purpose

   Mammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.

   Depositor

   Bas van Steensel

   Article

   Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

   Insert

   Cbx1 (Cbx1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam and V5

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter and heat shock minima…

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPV00554 pET21GG2-His(6)-BECN(248-450)

   Plasmid

   #136685

   Purpose

   Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 ECD/BARA (BECN1 Human)
   

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Plasmid

   #159577

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Insect

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

   Plasmid

   #159578

   Purpose

   Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Insect

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p426-Cup1p-FUS-FusionRed

   Plasmid

   #188393

   Purpose

   Cu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cells

   Depositor

   Michihiko Kataoka

   Article

   Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

   Insert

   FUS (FUS Human)
   

   Tags

   FusionRed

   Expression

   Yeast

   Promoter

   CUP1 promoter

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPV00605 pET21GG2-His(6)-BECN(1-265)

    Plasmid

    #136686

    Purpose

    Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 IDR + CC (BECN1 Human)
    

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP SRBC

    Plasmid

    #68399

    Purpose

    Expression of fluorescently tagged SRBC in mammalian cells

    Depositor

    Rob Parton

    Article

    Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

    Insert

    SRBC (Cavin3 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

    Plasmid

    #217012

    Purpose

    Expression of CDK7 in insect cells

    Depositor

    Manjeet Mukherjee

    Article

    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

    Insert

    CDK7 (S164D, T170E)_del327-346 (CDK7 Human)
    

    Tags

    TEV cleavable his tag

    Expression

    Insect

    Mutation

    (S164D, T170E)_del327-346

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

    Plasmid

    #217013

    Purpose

    Expression of CDK7 in insect cells

    Depositor

    Manjeet Mukherjee

    Article

    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

    Insert

    CDK7(W132R, S164D, T170E) (CDK7 Human)
    

    Tags

    TEV cleavable his tag

    Expression

    Insect

    Mutation

    CDK7(W132R, S164D, T170E)

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-Cx32D178Y-IRES-GCaMP6s

    Plasmid

    #216796

    Purpose

    Inducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

    Insert

    Cx32D178Y-IRES-GCaMP6s (GJB1 Human)
    

    Use

    Lentiviral

    Mutation

    D178Y mutant form of Connexin 32

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid

    #211799

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPG-UBI-Glis3M412

    Plasmid

    #114301

    Purpose

    Ubiquitous expression of a constitutively active form of the Glis3 transcription factor

    Depositor

    Patrick Salmon

    Insert

    Glis3 (Glis3 Mouse)
    

    Use

    Lentiviral

    Mutation

    N-terminal deletion up to Methionine 412

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-hPMCA4bct120

    Plasmid

    #47593

    Depositor

    Emanuel Strehler

    Insert

    PMCA4bct120 (ATP2B4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    120 C terminal AA deleted (constitutively active …

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLV-SFFV-mCherry-WPRE-UbC-MCS

    Plasmid

    #211801

    Purpose

    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    mCherry
    multiple cloning site
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-SFFV-MCS-WPRE-UbC-MCS

    Plasmid

    #211798

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    multiple cloning site
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-SFFV-MCS-WPRE-UbC-Emerald

    Plasmid

    #211796

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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