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Flag-MCPIP1(150-453)

Plasmid

#90109

Purpose

Mammalian expression of Flag-MCPIP1(150-453)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJAF210

Plasmid

#52406

Purpose

Expresses Flag-tagged mouse Arf5

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf5 (Arf5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pJAF214

Plasmid

#52404

Purpose

Expresses Flag-tagged mouse Arf3

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf3 (Arf3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pGG-PIP71

Plasmid

#196809

Purpose

Contains MYB15 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ARABIDOPSIS THALIANA MYB DOMAIN PROTEIN 15 (MYB15 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP29

Plasmid

#196767

Purpose

Contains GLR2.5 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

GLUTAMATE RECEPTOR 2.5 (GLR2.5 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

1488, 1489 G->C

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP01

Plasmid

#196739

Purpose

Contains PEP1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT ELICITOR PEPTIDE 1 (PROPEP1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(S1h)3(S3h)3-gs

Plasmid

#228079

Purpose

Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, S1h, S3h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(S1h-S3h)3-gs

Plasmid

#228076

Purpose

Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, S1h, S3h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-mock

Plasmid

#228504

Purpose

This plasmid can be used for control experiments and can also be used as a plasmid backbone

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-protein-mock

Plasmid

#228505

Purpose

This plasmid can be used for control experiments and can also be used as a plasmid backbone

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(D1h-D3h)3-gs

Plasmid

#228088

Purpose

Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, D1h, D3h

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)12-gs

Plasmid

#228087

Purpose

Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)3(S4h)3-gs

Plasmid

#228077

Purpose

Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h, S4h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

Plasmid

#200389

Purpose

3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

Depositor

Debojyoti Chakraborty

Insert

PX458_3XHA_dFnCas9_KRAB_eGFP

Use

CRISPR

Tags

3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

Expression

Mammalian

Promoter

CBH

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

Plasmid

#188272

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b(Q119F/E475R/E758R)

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTBL251 GG sgRNA

Plasmid

#126436

Purpose

To target a protospacer with GG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL510 CA sgRNA

Plasmid

#126430

Purpose

To target a protospacer with CA at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CA at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL401 GC sgRNA

Plasmid

#126435

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL071 AG sgRNA

Plasmid

#126428

Purpose

To target a protospacer with AG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge