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We narrowed to 2,616 results for: pCas

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Showing: 1381 - 1400 of 2616 results

  1. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid
    #154429
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Depositor
    David Liu
    Publication
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154429-plasmid-map-sequence-id-304585

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  2. YEE-BE4max

    Plasmid
    #138157
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YEE)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138157-plasmid-map-sequence-id-272348

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  4. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Publication
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  5. pX458-ECFP

    Plasmid
    #112220
    Purpose
    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP
    Depositor
    Yinming Liang
    Publication
    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112220-plasmid-map-sequence-id-215204

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  6. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  11. pCMV-PE-Plus

    Plasmid
    #214079
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214079-plasmid-map-sequence-id-429365

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  13. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139094-plasmid-map-sequence-id-273891

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  14. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131300-plasmid-map-sequence-id-254651

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  15. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  16. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  17. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  19. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  20. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Publication
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162277-plasmid-map-sequence-id-319794

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Showing: 1381 - 1400 of 2616 results